Determination of 20 Phthalate Acid Esters in Tea Leaves by GC-MS/MS

SUN Yu; ZHOU Li; ZHANG Xinfu; SUN Hezhi

doi:10.13305/j.cnki.jts.2025.04.007

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Journal of Tea Science ›› 2025, Vol. 45 ›› Issue (4) : 625-636. DOI: 10.13305/j.cnki.jts.2025.04.007

Research Paper

Determination of 20 Phthalate Acid Esters in Tea Leaves by GC-MS/MS

SUN Yu^1,2, ZHOU Li², ZHANG Xinfu^1,*, SUN Hezhi^2,*

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Abstract

In this paper, an analytical method for the simultaneous determination of 20 phthalate esters in tea fresh leaves was developed by gas chromatography-tandem mass spectrometry (GC-MS/MS). The phthalate esters in tea fresh leaves were extracted by 0.1% acetonitrile acetate, salted out with sodium chloride, cleaned up with a florisil solid phase extraction column, resolved with acetonitrile, separated by temperature programmed chromatography, monitored with multiple reaction monitoring (MRM) mode, and quantified by the external standard method. Through the optimization of sample extraction, purification and chromatographic conditions, good linearity of the target compounds was obtained in the concentration range of 0.010-2.0 mg·kg^-1 with the corresponding coefficient of determination R²>0.995 5. The average recoveries ranged from 70% to 118% at the three spiked levels of 0.010 mg·kg^-1, 0.10 mg·kg^-1 and 1.0 mg·kg^-1, with the relative standard deviations (RSDs) of <17.8%. The limits of quantification were 0.010~0.050 mg·kg^-1. The method is stable and reliable, and it can meet the needs of the determination of phthalate acid esters in tea fresh leaves.

Key words

gas chromatography-tandem mass spectrometry / phthalate acid esters / tea fresh leaves

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SUN Yu, ZHOU Li, ZHANG Xinfu, SUN Hezhi. Determination of 20 Phthalate Acid Esters in Tea Leaves by GC-MS/MS[J]. Journal of Tea Science. 2025, 45(4): 625-636 https://doi.org/10.13305/j.cnki.jts.2025.04.007

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