The effects of green tea polyphenols on the malformations of the fertilized eggs of Japanese medaka (Oryzias latipes) induced by benz[a]pyrene was investigated by using the biological model of spawning Japanese medaka (Oryzias latipes) with the method of intraperitoneal injection, and the real-time PCR was used to detection the mRNA expression of CYP enzymes (CYP1A1, CYP1B, CYP2A, CYP2C, CYP2D, CYP3A) and the GST gene (mGST, GST-a) in the liver medaka. The results showed that the malformation rate of the fertilized eggs of Japanese medaka in the treatment of injection of benzo[a]pyrene which was significantly higher than the DMSO control. The malformation rate in the treatments of tea polyphenols was decreased, the treatments except the lowest dose of tea polyphenols (20μg/g BaP +2μg/g TP) were significantly different compared to the BaP treatment. Real-time quantitative PCR results showed that the benzo[a]pyrene model group slightly induces the CYP1A1, CYP1BCYP2A, CYP2C, CYP2D mRNA expression and significantly inhibits the mGST and GST-a mRNA expression of the female medaka, while tea polyphenols can significantly inhibit the expression CYP1A1 mRNA gene and glutathione-S transferase (GST) gene mGST and GST-a. The antagonistic mechanism of green tea polyphenols on the malformation of the fertilized eggs of Japanese medaka induced by benzo[a]pyrene might be correlated with the inhibition of CYP1A1 mRNA gene expression and the acceleration of the excretion of metabolic product of benzopyrene.
SONG Chuan-kui
,
XIAO Bin
,
WANG Yan-li
,
YU You-ben
,
ZHOU Tian-shan
. The Antagonistic Effects of Green Tea Polyphenols on the Malformations of the Fertilized Eggs of Japanese Medaka (Oryzias latipes) Induced by Benzo[a]pyrene[J]. Journal of Tea Science, 2011
, 31(3)
: 255
-263
.
DOI: 10.13305/j.cnki.jts.2011.03.001
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