茶多酚对苯并[a]芘致青鳉鱼后代发育畸形的拮抗作用

doi:10.13305/j.cnki.jts.2011.03.001

Abstract

Abstract: The effects of green tea polyphenols on the malformations of the fertilized eggs of Japanese medaka (Oryzias latipes) induced by benz[a]pyrene was investigated by using the biological model of spawning Japanese medaka (Oryzias latipes) with the method of intraperitoneal injection, and the real-time PCR was used to detection the mRNA expression of CYP enzymes (CYP1A1, CYP1B, CYP2A, CYP2C, CYP2D, CYP3A) and the GST gene (mGST, GST-a) in the liver medaka. The results showed that the malformation rate of the fertilized eggs of Japanese medaka in the treatment of injection of benzo[a]pyrene which was significantly higher than the DMSO control. The malformation rate in the treatments of tea polyphenols was decreased, the treatments except the lowest dose of tea polyphenols (20μg/g BaP +2μg/g TP) were significantly different compared to the BaP treatment. Real-time quantitative PCR results showed that the benzo[a]pyrene model group slightly induces the CYP1A1, CYP1BCYP2A, CYP2C, CYP2D mRNA expression and significantly inhibits the mGST and GST-a mRNA expression of the female medaka, while tea polyphenols can significantly inhibit the expression CYP1A1 mRNA gene and glutathione-S transferase (GST) gene mGST and GST-a. The antagonistic mechanism of green tea polyphenols on the malformation of the fertilized eggs of Japanese medaka induced by benzo[a]pyrene might be correlated with the inhibition of CYP1A1 mRNA gene expression and the acceleration of the excretion of metabolic product of benzopyrene.

Key words: tea polyphenols, benzo[a]pyrene, malformations, antagonistic

CLC Number:

SONG Chuan-kui, XIAO Bin, WANG Yan-li, YU You-ben, ZHOU Tian-shan. The Antagonistic Effects of Green Tea Polyphenols on the Malformations of the Fertilized Eggs of Japanese Medaka (Oryzias latipes) Induced by Benzo[a]pyrene[J]. Journal of Tea Science, 2011, 31(3): 255-263.

References

[1] Reynaud S, Deschaux P.The effects of polycyclic aromatic hydrocarbons on the immune system of fish: A review[J]. Aquatic Toxicology, 2006, 77: 229-238.
[2] 刘宇红, 于晓英, 韩宁. 苯并[a]芘(BaP)的毒性作用与致毒机理研究现状[J]. 内蒙古农业大学学报, 2008, 29(1): 184-188.
[3] 邓南圣, 吴峰. 环境中的内分泌干扰物[M]. 北京: 化学工业出版社, 2004: 7-10.
[4] 张星海, 王岳飞. 茶多酚与绿原酸生物活性的比较研究[J]. 茶叶科学, 2007, 27(1): 39-44.
[5] 刘本英, 王平盛. 茶多酚生物学活性的研究进展[J]. 中国农学通报, 2005, 21(12): 92-94.
[6] Medhumita R, Sutapa C, Dona S, et al. Antielastogenic, antiegenotoxic and apoptotic activity of epigallocateehin gallate:a green tea polyphenol[J]. Mutat Res, 2003(523/524): 33-41.
[7] Yukiaki K, Yukihiko H.Antimutagenic and anticarcinogenic activity of tea polyphenols[J]. Mutation Research, 1999,436: 69-97.
[8] Yang X F, Wang N P, Zeng F D.Effects of the active components of some Chinese herbs on drug metabolizing enzymes[J]. China J Chin M atMed, 2002, 27(5): 325-328.
[9] McBride M W, Brosnan M J, Mathers J, et al. Reduction of Gstm expression in the stroke prone spontaneously hypertension rat contributes to increased oxidative stress[J]. Hypertension, 2005, 45(4): 786-792.
[10] Liu J, Lei D, WaalkesM P, et al. Genomic analysis of the rat lung following elementalmercury vapor exposure[J]. Toxicol Sci, 2003, 74(1):174-181.
[11] Yang X F, Wang N P, LuW H, et al. Effects of Ginkgo biloba extract and tanshinone on cytochrome P-450 isozymes and glutathione transferase in rats[J]. Acta Pharm acol S in, 2003, 24(10): 1033-1038.
[12] Kristine L W, Shobana G, Monali P, et al. In vivo and in vitro CYP1B mRNA expression in channel catfish[J]. Marine Environmental Research, 2006, 62(S1): S332-S336.
[13] Carlson E A, Li Y, Zelikoff J T.Exposure of Japanese medaka (Oryzias latipes) to benzo[a]pyrene suppresses immune function and host resistance against bacterial challenge[J]. Aquatic Toxicology , 2002, 56(4): 289-301.
[14] 李龙, 陈家堃. 现代毒理学实验技术原理与方法[M]. 北京: 化学工业出版社, 2006: 301-302.
[15] Zhang Z B, Hu J Y.Development and validation of endogenous reference genes for exp ression p rofiling of medaka (Oryzias latipes) exposed to endocrine disrup ting chemicals by quantitative real time RT-PCR[J]. Toxicological Sciences, 2007, 95(2): 356-368.
[16] 杨贤强, 王岳飞, 陈留记, 等. 茶多酚化学[M]. 上海:上海科技出版社, 2003: 59, 127-151.
[17] 丁仁凤, 何普明, 揭国良. 茶多糖和茶多酚的降血糖作用研究[J]. 茶叶科学, 2005, 25(3): 219-224.
[18] 赵保路. 茶多酚保护脑神经防止帕金森病损伤作用及其分子机理[J]. 生物化学和生物物理进展, 2008, 35(7): 735-743.
[19] Sang I L, Hyo J K, Yong C B.Effect of Green Tea and (-)-Epigallocatechin Gallate on Ethanol-induced Toxicity in HepG2 Cells[J]. PHYTOTHERAPY RESEARCH Phytother Res, 2008, 22(5): 669-674.
[20] H C Kim, E J Chang, K C Mun.Effects of Epigallocatechin Gallate on the Hemolysis Induced by Cyclosporine[J]. Transplantation Proceedings, 2005, 37(5): 2385-2386.
[21] Akihiro Higuchi, Kosei Yonemitsu, Ako Koreeda, et al. Inhibitory activity of epigallocatechin gallate (EGCg) in paraquat-induced microsomal lipid peroxidation—a mechanism of protective effects of EGCg against paraquat toxicity[J]. Toxicology, 2003, 183(1/2/3): 143-149.
[22] Sikandar G, Khan, Santosh K, et al. Enhancement of antioxidant and phase II enzymes by oral feeding green tea polyphenols in drinking water to SKH21 hairless mice: possible role in cancer chemop revent ion[J]. Cancer Res, 1992, 52(14): 4050-4052.
[23] Manal E A, Elhalwagy a, Nevine S, et al. Prophylactic effect of green tea polyphenols against liver and kidney injury induced by fenitrothion insecticide[J]. Pesticide Biochemistry and Physiology, 2008, 91(2): 81-89.
[24] Li D, Wang M, Dhingra K, et al. Modulation of the mutagenic activity of cigarette smoke,cigarette smoke condensate and benzo(a)pyrene in vitro and in vivo[J]. Cancer Res, 1996, 56(2): 287-293.
[25] 冯磊, 余应年, 陈星若. 茶多酚对人羊膜上皮细胞FL系CYP1A 1mRNA水平和转录的影响[J]. 癌变·畸变·突变, 1996, 8(6): 332-336.
[26] Salassidis K, Kulka U, Schmid E, et al. Induction of chromosome aberrations and sister chromatid exchange by indirectly acting mutagens in immortal mouse and rat hepatocyte lines[J]. Mutagenesis, 1991, 6(1): 59-63.
[27] Willems MI, Roggeband R, Baan RA, et al. Monitoring the exposure of rats to benzo(a)pyrene by the determination of mutagenic activity in excreta,chromosome aberrations and sister chromatid exchanges in peripheral blood cells,and DNA adducts in peripheral blood lymphocytes and liver[J]. Mutagenesis, 1991, 6(2): 151-158.

The Antagonistic Effects of Green Tea Polyphenols on the Malformations of the Fertilized Eggs of Japanese Medaka (Oryzias latipes) Induced by Benzo[a]pyrene

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