Based on AFLP method, AFLP figure were obtained in Guangdong tea plants. In this study, totally 365 bands were amplified by 5 pairs of primes and 73 bands were amplified by each primer pair on average, a total of 338 (92.6%) polymorphic AFLP markers were detected. The results indicated that the genetic distance (GD) based on AFLP data by using DPS 2000 was significant. The GD(0.5) is highest between Guibeidaye and Fenghuangshuixian, but the GD(0.12752) between Qingliangshan tea and Qingyuanbijia tea is low. This indicated that the Guangdong tea plant has a high genetic diversity.
ZHAO Chao-yi
,
LUO Jun-wu
,
HUANG Jian-an
,
TAN He-ping
. AFLP Analysis of Genetic Diversity of Tea plant Germplasms in Guangdong Province[J]. Journal of Tea Science, 2006
, 26(4)
: 249
-252
.
DOI: 10.13305/j.cnki.jts.2006.04.003
[1] Vos P.AFLP: a new technique for DNA fingerprint[J]. Nucl, Acids, Res, 1995(23): 4407~4414.
[2] M Labra, T Di Fabio, F Grassi, et al. AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants[J]. Chemosphere, 2003(52): 1183~1188.
[3] Katia Gil-Vega, Corina Dı´az, Alejandro Nava-Cedillo, ,et al. AFLP analysis of Agave tequilana varieties[J]. Plant Science. AFLP analysis of Agave tequilana varieties[J]. Plant Science, 2006(170): 904~909. [下转第258页]