Obtain the optimum reaction condition of synthesizing theaflavins by enzymatic oxidation, synthesizing theaflavins was studied by raw ppo extracted from fresh tea leaves under single factor and orthogonal experimental design. Based on these results, synthesizing theaflavins was also studied by using breaking fresh tea leaves. The result showed that the optimum condition of theaflavins synthesizing is 30℃ temperature is, under this reaction system, pH value of the present system is 4.8, the concentration of substrates is 0.9βg/100βml, the concentration of enzymes is 28βg/100βml (based on the fresh weight), oxygen rate is 0.4βL/min. Temperatures and pH values in the reaction system are the most important factors(P<0.05), and adding suitable oxygen is another necessary factor in theaflavins synthesis by enzymatic oxidation.
GU Ji-ping
,
LIU Zhong-hua
,
HUANG Jian-an
,
SHI Zhao-peng
. Study on the Optimum Condition of Synthesizing Theaflavins by Using Enzyme-catalysing Oxidation[J]. Journal of Tea Science, 2006
, 26(4)
: 285
-290
.
DOI: 10.13305/j.cnki.jts.2006.04.010
[1] Bacus, James V, Kelloff, et al. Chemoprevention with theaflavins of rat esophageal intraepithelial neoplasia quantitatively monitored by image tile analysis.Cancer epidemiology,biomarkers & prevention[M]. A pubication of the American association for cancer research / cosponsored by the American society of preventive oncology, 2000, 9(11): 1149~1154.
[2] Eng, Qing, Torii, et al. Black tea polyphenols, theaflavins,prevent celluar DNA damige by inhibiting oxidative stress and suppressing cytochrome P450 1A1 in cell cultures[J]. Journal of Agricultrual and food chemistry, 2002, 50(1): 213~220.
[3] Uptas.Anticlastogenic effects of black tea (World blend) and its tow active polyphenols theaflavins and thearubigins in vivo in Swiss albion mice[J]. Life science, 2001, 69(23): 2735~2744.
[4] 大泽俊彦. 应用植物成分进行化学防癌[J]. 日本医学介绍, 2004, 25(1): 26~27.
[5] Cong YY, Han C, Chen JS.Effect of tea polyphenols and tea pigments on the inhibition of precancerous 1iver lesions in rats[J]. Nutr And Cancer, 2000, 38(1): 81~86.
[6] Lin Yu-li, Tsai Shu-huei, Lin-shiau, et a1, Theaflavin-3,3’ -digallate from black tea the nitric oxide synthase by down-regulating the actination of NF-kappa Bin macrophages[J]. Europen J of pharmacology, 1999, 367(2~3): 379~388.
[7] 范远景, 张劲松, 高学云. 茶水提取物和茶多酚抑制诱变的类型及其机制, 茶·癌变·畸变·突变[J]. 2003, 15(3): 153~156,167.
[8] 王坤波, 刘仲华, 黄建安. 儿茶素体外氧化制备茶黄素的研究[J]. 茶叶科学, 2004, 24(1): 53~59.
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