Theaflavin digallate was purified by Sephadex LH-20 column chromatography. Its anticancer activity and mechanism were analyzed on H1299 and HCT-116 cancer cell lines. Results from MTT assay showed that Theaflavin digallate could inhibit the growth of H1299, and its IC50 value is 25μmol/L. Theaflavin digallate could slightly adjust the cell cycle of HCT-116 cancer cell, and significantly promote the apoptosis of HCT-116 cancer cell at 50μmol/L. Theaflavin digallagte could decrease the expression of cancer-promoting biomarker, Bcl-xL, and increase the expression of cancer-inhibiting biomarker, Bax, on HCT-116 cancer cell line.
JIANG He-yuan
,
Hang Xiao
,
YUAN Xin-yue
,
WANG Chuan-pi
,
Chung S Yang
. Anticancer Activity of Theaflavin Diagallate and its Mechanism[J]. Journal of Tea Science, 2007
, 27(1)
: 33
-38
.
DOI: 10.13305/j.cnki.jts.2007.01.005
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