The preparative technology of theanine from the aqueous phase of tea polypheonls production was investigated. Firstly, retreating the aqueous liquids with ZTC-II, then, Separating the clarifying liquids by three kinds of ultra-filtration, the crude theanine (>60%) can be obtained from the filtrated liquid by using the JAD-2000 which is a kind of macroporous resin with weak polarity. High purity theanine (>98%) can be obtained through preparative high performance liquid chromatography (prep-HPLC) with C18 column. Results showed that proteins and pectin compounds were removed over 97% and 89% respectively, after flocculating and ultra-filtration. The removing rate of tea polypheonls and soluble sugar was less than 9% and the theanine was reserved above 93%. Crude theanine and high purity theanine were produced through JAD-2000 and the C18 column for prep-HPLC, the final recovering rate and purity of theanine were 71.5%, 61.2% and 71.3%, 98.3% respectively.
ZHANG Xing-hai
,
ZHOU Xiao-hong
,
LU Xuan
,
WANG Yue-fei
. Study on the Isolation Technology of Theanine from Aqueous Phase of Tea Polypheonls Production[J]. Journal of Tea Science, 2008
, 28(6)
: 443
-449
.
DOI: 10.13305/j.cnki.jts.2008.06.007
[1] Juneja L R, DC Chu, T Okubo, et al. L-theanine-a unique amino acid of green tea and its relaxation effect in human[J]. Trends in Food Science & Technology, 1999,10:199-204
[2] Terashima T, J Takido, H Yokogoshi, et al. Time-dependent changes of amino acids in the serum, liver, brain and urine of rats administered with theanine[J]. Biosci.Biotech.Biochem, 1999,63(4): 615-618
[3] H Yokogoshi,, M Kobayashi, M Mochizuki, et al. Effect of theanine, r-glutamylethylamide, on brain monoamines and striatal dopamine release and kinds of behavior in rats[J]. Nutrition, 2000,16(9): 776
[4] Y Sadzuka, T Sugiyama, T Sonobe, et al. Improvement of idarubicin induced antitumor activity and bone macro suppression by theanine, a component of tea[J]. Cancer Lett.2000,156:199~124.
[5] 朱松, 王洪新. 茶氨酸的生理功能及在食品中的应用. 食品研究与开发, 2005, 26(3): 18~20.
[6] 吕毅, 郭雯飞, 倪捷儿, 等. 茶氨酸的生理作用与合成[J]. 茶叶科学, 2003, 23(1): 1~5.
[7] 张莹, 施兆鹏. 色谱法制备茶氨酸的研究[J]. 茶叶科学, 2004, 24(4): 283~287.
[8] 林智, 杨勇, 谭俊峰, 等茶氨酸提取纯化工艺研究[J]. 天然产物研究与开发, 2004, 116(5): 442~447.
浙公网安备 33019902000101号 
