茶多酚生产水相中茶氨酸分离技术研究

doi:10.13305/j.cnki.jts.2008.06.007

Abstract

Abstract: The preparative technology of theanine from the aqueous phase of tea polypheonls production was investigated. Firstly, retreating the aqueous liquids with ZTC-II, then, Separating the clarifying liquids by three kinds of ultra-filtration, the crude theanine (>60%) can be obtained from the filtrated liquid by using the JAD-2000 which is a kind of macroporous resin with weak polarity. High purity theanine (>98%) can be obtained through preparative high performance liquid chromatography (prep-HPLC) with C₁₈ column. Results showed that proteins and pectin compounds were removed over 97% and 89% respectively, after flocculating and ultra-filtration. The removing rate of tea polypheonls and soluble sugar was less than 9% and the theanine was reserved above 93%. Crude theanine and high purity theanine were produced through JAD-2000 and the C₁₈ column for prep-HPLC, the final recovering rate and purity of theanine were 71.5%, 61.2% and 71.3%, 98.3% respectively.

Key words: theanine, production waste-liquor, ZTC, ultrafiltration, macroporous resin

CLC Number:

ZHANG Xing-hai, ZHOU Xiao-hong, LU Xuan, WANG Yue-fei. Study on the Isolation Technology of Theanine from Aqueous Phase of Tea Polypheonls Production[J]. Journal of Tea Science, 2008, 28(6): 443-449.

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Study on the Isolation Technology of Theanine from Aqueous Phase of Tea Polypheonls Production

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