Research on Synergistic Protection and Repairing Effects of EGCG and Theanine in Oxidative Damaged Cells

LIU Xiaohui; JIE Guoliang; LIN Kang; WU Yuanyuan; WANG Yuefei; TU Youying

doi:10.13305/j.cnki.jts.2014.03.005

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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (3) : 239-247. DOI: 10.13305/j.cnki.jts.2014.03.005

Research on Synergistic Protection and Repairing Effects of EGCG and Theanine in Oxidative Damaged Cells

LIU Xiaohui¹, JIE Guoliang¹, LIN Kang², WU Yuanyuan¹, WANG Yuefei¹, TU Youying^1,*

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Abstract

This paper investigated the scavenging capacities of EGCG and theanine, the two main active components in tea, alone or synergistically on ABTS, DPPH and hydroxyl radicals in vitro were investigated. The oxidative injury experimental model of mouse macrophage (RAW 264.7) by H₂O₂ was established for measuring the oxidative level and antioxidative enzyme activities by EGCG and theanine. The results showed that EGCG and theanine showed synergistic effect on the scavenging of ABTS and hydroxyl radical except for the DPPH radical. EGCG and theanine could synergistically reduce the contents of reactive oxygen species and lipid peroxidation, and enhance the activity of GSH-Px on RAW 264.7, which meant that the combination of above two compounds could synergistically reduce the oxidative damage of the cells through improving the antioxidant capacity.

Key words

antioxidant / EGCG / synergistic effect / theanine

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LIU Xiaohui, JIE Guoliang, LIN Kang, WU Yuanyuan, WANG Yuefei, TU Youying. Research on Synergistic Protection and Repairing Effects of EGCG and Theanine in Oxidative Damaged Cells[J]. Journal of Tea Science. 2014, 34(3): 239-247 https://doi.org/10.13305/j.cnki.jts.2014.03.005

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