Rapid Detection of Theanine in Green Tea Based on Direct-reading Colorimetric Strips

ZHU Chengbo; WU Xiaomei; FENG Lixia; HUANG Xiangpeng; PANG Linjiang; YANG Huqing; FANG Zhengwei; WANG Ying; LAI Shuyu; ZHANG Yiming

doi:10.13305/j.cnki.jts.2018.03.004

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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (3) : 244-252. DOI: 10.13305/j.cnki.jts.2018.03.004

Rapid Detection of Theanine in Green Tea Based on Direct-reading Colorimetric Strips

ZHU Chengbo¹, WU Xiaomei¹, FENG Lixia¹, HUANG Xiangpeng¹, PANG Linjiang¹, YANG Huqing¹, FANG Zhengwei¹, WANG Ying¹, LAI Shuyu¹, ZHANG Yiming^1,2,*

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Abstract

In this study, ninhydrin/titanium dioxide nanomaterials were constructed by using Sol-Gel method. These nanomaterial were then used as direct-reading colorimetric materials and printed on the surface loci of thin layer plate where theanine would migrate according to its retardation factor (R_f). In addition, ethanol: acetic acid developing system was chosen as developing reagent, the extraction solution of tea after being decolorized by ethyl acetate was analyzed by thin layer chromatography (TLC) via direct-reading colorimetric reaction. The results showed that the current direct-reading strip could effectively separate several amino acids in tea extraction under optimized conditions. The new constructed colorimetric nanomaterials had achieved a sensitive direct-reading coloration results for the target amino acid theanine, and the color spots on the strip were uniform and clear after self-coloration. Moreover, its color depth had a clear semi-quantitative relationship with its concentration. The current colorimetric strip has a serial of advantages including safety, easy-operation, convenient and low cost, and has a broad application prospect in fields such as quality identification of agricultural products and on-site rapid detection of amino acids.

Key words

direct-reading colorimetric strips / green tea / theanine / thin layer chromatography

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ZHU Chengbo, WU Xiaomei, FENG Lixia, HUANG Xiangpeng, PANG Linjiang, YANG Huqing, FANG Zhengwei, WANG Ying, LAI Shuyu, ZHANG Yiming. Rapid Detection of Theanine in Green Tea Based on Direct-reading Colorimetric Strips[J]. Journal of Tea Science. 2018, 38(3): 244-252 https://doi.org/10.13305/j.cnki.jts.2018.03.004

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