Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (5): 484-490.doi: 10.13305/j.cnki.jts.2016.05.006

Previous Articles     Next Articles

Study on the Protective Effect of L-Theanine on the Liver of Mice with Immune Stress Induced by ETEC

LIU Qiuling1,2, GONG Zhihua1,2, CHENG Ling1,2, LIU Zunying1,2, DENG Yanli1,2, CHEN Dong1, XIAO Wenjun1,2,3,*   

  1. 1. Key Lab of Tea Science of Ministry of Education, Changsha 410128, China;
    2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    3. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China
  • Received:2016-01-20 Online:2016-10-15 Published:2019-08-26

Abstract: SPF grade Balb/c female mice were used for this experiment. After adaptive feeding for 3 d, the mice were pre-treated orally with different dosages of L-theanine once daily for 30 d. ETEC E44813 intraperitoneal injection was subsequently performed and samples were collected 5h later. The coefficient of liver and spleen, levels of serum alanine aminotranferease (ALT), aspartate aminotransferase (AST) as well as liver homogenate malondialdehyde (MDA), and activities of liver homogenate superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), pathological changes of liver, expressions of serum γ-Interferon (IFN-γ) and Interleukin-4 (IL-4) were studied to explore the protective effects of L-theanine on liver of ETEC immune mouse model. Results show that pretreatment with L-theanine significantly reduced the increase of liver and spleen coefficient caused by ETEC infection|lowered the levels of serum ALT|AST and liver homogenate MDA|increased the activities of liver homogenate SOD|CAT|GSH-Px|decreased the expressions of serum IFN-γ|IL-4|and improved pathological injury of liver tissue. Therefore|it can be concluded that L-theanine is able to prevent ETEC-induced hepatic injury through depressing IFN-γ, IL-4 and inflammatory reaction and enhancing hepatocyte antioxidant abilities.

Key words: L-theanine, enterotoxigenic Escherichia coli E44813, immune stress, liver protection

CLC Number: