酶法拆分DL-茶氨酸及其分离纯化

doi:10.13305/j.cnki.jts.2006.01.005

Abstract

Abstract: An enzymatic method for the optical resolution of DL-theanine by aminoacylases from Cunnighamella echinulata 9980 was developed. The optimum temperature, pH and substrate concentration were: 50℃, pH7.0, and 0.5mol/L N-Ac-DL-theanine. The conversion rate against N-Ac-L-theanine was 92%. Productions were purified on JK008 ion exchange resin. The yield of L-theanine was 84.3%, [α] =8.1(c=2,H₂O), complied with JP2000。

Key words: L-theanine, N-Ac-DL- theanine, Cunnighamella echinulata 9980, aminoacylase, optical resolution

CLC Number:

GUO Li-yun, LIU Yi, JIA Xiao-juan, LI Zhao-lan, JIAO Qing-cai. Resolution of DL-theanine by Aminoacylase and the Separation of Productions[J]. Journal of Tea Science, 2006, 26(1): 31-36.

References 14

[1]	Nakagawa M.Constituents in tea leaf and their contribution to the taste of green tea liquor[J]. Jpn Agric Res Q. 1970, 5: 43~47.
[2]	Yokogoshi H, Kobayashi M.Hypotensive effect of γ-glutamylmethylamide in spontaneously hypertensive rats[J]. Life Sciences Including Pharmacology Letters. 1998,62(12):1065~1068.
[3]	Juneja L R, Chu D C, Okubo T, et al.L-theanine-a unique amino acid of green tea and its relaxation effect in humans[J]. Trends in Food Science &Technology. 1999, 10(6~7): 199~204.
[4]	Sugiyama T, Sadzuka Y.Enhancing effects of green tea components on the antitumor activity of adriamycin against M5076 ovarian sarcoma[J]. Cancer Letters. 1998, 133(1): 19~26.
[5]	Sadzuka Y, Sugiyama T, Miyagishima Atsuo et al. The effects of theanine as a novel biochemical modulator on the antitumor activity of adriamycin[J]. Cancer Letters. 1996, 105(2): 203~209.
[6]	Sadzuka Y, Sugiyama T, Sonobe T.Efficacies of tea components on doxorubicin induced antitumor activity and reversal of multidrug resistance[J]. Toxicology Letters. 2000, 114(1~3): 155~162.
[7]	Kakuda T, Yanase H, Utsunomiya K, et al.Protetive effect of γ-glutamylethylamide (theanine) on ischemic delayed neuronal death in gerbils[J]. Neurosci Lett. 2000, 289: 189~192.
[8]	李炎, 王秀芬, 刘锋, 等. 茶氨酸的合成方法[P].2003, CN 1415599A.
[9]	Furuyama T. synthesis of L-theanine[J]. Bull Chem Soc Jap. 1964(37): 1078.
[10]	Tachiki T.Production of γ-glutamylmethylamide and γ-glutamylethylamide by coupling of baker’s yeast preparations and bacterial glutamine synthetase[J]. J gen. Appl Microbiol. 1986(32): 545~548.
[11]	Matsura Tl.Effects of precursor, temperature, and illumination on theanine accumulation in tea callus[J]. Agric Biol Chem. 1990(54): 2283~2286.
[12]	Orihara T.Production of theanine and other γ-glutamyl derivatives by camellia sinensis cultured cells[J]. Plant Cell Rep. 1990, 9: 65~68.
[13]	Tachiki T.γ-glutamyl transfer reactions by glutaminase from psendomoas nitroreducens IFO 12694 and their application for the synthesis of theanine and γ- glutamylmethylamide[J]. Biosci Biotechnol Biochem. 1998(62): 1279~1283.
[14]	Suzuki H.Enzymatic production of theanine, an “umami” component of tea, from glutamine and ethylamine with bacterial-glutamyl transpeptidase[J]. Enzyme and Microbiol Technology. 2000, 31: 884~889.

Resolution of DL-theanine by Aminoacylase and the Separation of Productions

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 14

Related Articles 7

Metrics

Comments

Recommended 10

[1]	ZENG Li, XIAO Wenjun. Advances in Brain Diseases Prevention and Treatment of L- theanine [J]. Journal of Tea Science, 2019, 39(2): 193-202.
[2]	PENG Yingqi, XIAO Wenjun, ZHANG Sheng, YUAN Dongyin, LIN Ling, ZHOU Yang, GONG Zhihua. Effects of L-theanine on Intestinal Morphology and Free Amino Acids in Mice [J]. Journal of Tea Science, 2019, 39(1): 43-54.
[3]	GUO Chen, LIU Linfeng, XIANG Yi, LUO Li, LIN Ling, XIAO Wenjun. L-theanine Attenuates Tissue and Oxidative Damages in Heat Stressed Mice [J]. Journal of Tea Science, 2017, 37(1): 17-24.
[4]	LIU Zunying, LIU Qiuling, DENG Yanli, CHEN Ling, XIAO Wenjun. Study on the Protective Effect of L-Theanine on the Intestinal in Mice with Immune Stress Induced by ETEC [J]. Journal of Tea Science, 2016, 36(5): 469-476.
[5]	LIU Qiuling, GONG Zhihua, CHENG Ling, LIU Zunying, DENG Yanli, CHEN Dong, XIAO Wenjun. Study on the Protective Effect of L-Theanine on the Liver of Mice with Immune Stress Induced by ETEC [J]. Journal of Tea Science, 2016, 36(5): 484-490.
[6]	PENG Bin, LIU Zhonghua, LIN Yong, LIN Haiyan, HUANG Jian'an. The Ameliorative Effect of L-theanine on Chronic Unpredictable Mild Stress-induced Depression in Rats [J]. Journal of Tea Science, 2014, 34(4): 355-363.
[7]	CHEN Lin, WAN Xiao-chun, ZHANG Zheng-zhu, CHEN Qi. Distribution of Catechins, Purine Alkaloids and Free Amino Acids in Tea Seedlings [J]. Journal of Tea Science, 2008, 28(1): 43-49.