Six compounds separated from Fuzhuan Tea were tested by High-Throughput Screening of model FXR, LXR, PPARδ, PPARγ and 3T3-L1 related to the lipid-depressing and anti-obesity. Results showed that all monomers were active to the models selected. To the active model FXR, gallic acid and ECG were active on the concentration of 50 µg/ml. The activating value of GA and ECG was 1.766 and 3.220 respectively. EGCG was very active on the concentration of 10 µg/ml and 50 µg/ml. The active value reached 6.0 when the concentration was 50 µg/ml. To PPARδ, the three compounds were active when the concentration was 50 µg/ml. The activating value of GC to PPARγ model was 1.619. The activating value of 3- methoxy- 4,5-dihydroxy-benzoic acid in PPARγ model was 1.734. The restraining value of 3,4-dihydroxy-benzoic acid in FXR model was 3.641, while that of CDCA(CK) was 6.435. All the results showed that all the compounds were functional ones.
FU Dong-he
,
LIU Zhong-hua
,
HUANG Jian-an
,
CHEN Jin-hua
,
CHEN Hui-heng
. Investigation on the Function of Monomers in Fuzhuan Tea by High-Throughput Screening[J]. Journal of Tea Science, 2008
, 28(1)
: 39
-42
.
DOI: 10.13305/j.cnki.jts.2008.01.006
[1] 傅冬和, 刘仲华, 黄建安, 等. 高通量筛选研究茯砖茶降脂减肥功效[J]. 茶叶科学, 2006, 26(3): 209~214.
[2] 傅冬和, 刘仲华, 黄建安, 等. 高通量筛选研究茯砖茶对FXR的作用[J]. 食品科学. 2007, 28(5): 331~334.
[3] Vosper H, Patel L, Graham T L, et al. The peroxisome proliferators-activate receptor[delta] promotes lipid accumulation in human macrophages[J]. J Biol Chem, 2001, 276: 44258
[4] Lee C H, Chawla A, Urbiztondo N, et al. Transcriptional repression of atherogenic inflammation: modulation by PPAR-delta[J]. Science, 2003, 302: 4553~457
[5] Frohnert B I, Hui T Y, Bernlohr DA.Identification of functional peroxisome proliferator-responsive element in murine fatty acid transport protein gene[J]. J Biol Chem, 1999, 274: 3970~3977.
[6] Lapsys N M, Kriketos A D, Lim-Faster M, et al. Expression of genes involved in lipid metabolism correlate with peroxisome proliferators-activated receptor γ expression in human skeletal muscle[J]. Clin Endocrinol Metab, 2000, 85: 4293~4297.
[7] Lehmann J M, Kliewer S A, Moore L B, et al.Activation of the nuclear receptor LXR by oxysterols defines a new hormone response pathway[J]. J Biol Chem, 1997,272:3137~3140.
[8] Schultz J R, Tu H, Luk A, et al. Role of LXRs in control of lipogenesis[J]. Genes Dev, 2000, 14: 2831~2838.
[9] 李烁, 张志文, 管又飞. 胆汁酸受体FXR的研究进展[J].生理科学进展. 2003, 34(4): 314~318.
[10] Urizar N L, Liveryman A B, Dodd’s DT, et al. A natural product that lowers cholesterol as antagonist ligand for FXR[J]. Science, 2002, 296: 1703~1706.
[11] 肖文军, 傅冬和, 任国谱, 等. 茯茶毒理学试验报告[J]. 茶叶科学. 2007, 27(4): 307~310.
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