A novel derivative method for determination of theanine was developed by micellar electrokinetic capillary chromatography (MECC). Samples were derivated with 2,4-dinitrofluorobenzen. The buffer solution used in this method was 0.03mol/L sodium borate solution (pH9.8). Column temperature was 17℃. The separating voltage was 28βkV and detection at 360βnm. Factors of pH value, reaction time, reaction temperature and concentration of buffer were optimized. The products of derivation of theanine can be stable for five days at 4℃ or 25℃. The linearity of this assay was 0.2~5βmmol/L (r2=0.993). The minimal detection limit (S/N=2) was 0.05βmmol/L.
LI Ping
,
WAN Xiao-chun
,
LI Jian
,
ZHANG Zheng-zhu
,
SHEN Zuo-jun
. Derivation of Theanine and Its Determination by Capillary Electrophoresis[J]. Journal of Tea Science, 2004
, 24(2)
: 119
-123
.
DOI: 10.13305/j.cnki.jts.2004.02.009
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