Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2004, Vol. 24 ›› Issue (2): 119-123.doi: 10.13305/j.cnki.jts.2004.02.009

Previous Articles     Next Articles

Derivation of Theanine and Its Determination by Capillary Electrophoresis

LI Ping1, WAN Xiao-chun1, LI Jian1, ZHANG Zheng-zhu1, SHEN Zuo-jun2   

  1. 1. Anhui Agricultural University, Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Agriculture, Hefei 230036, China;
    2. Anhui Provincial Hospital, Anhui Provincial Center for Clinical Laboratory, Hefei 230001, China
  • Received:2003-10-24 Online:2004-06-25 Published:2019-09-16

Abstract: A novel derivative method for determination of theanine was developed by micellar electrokinetic capillary chromatography (MECC). Samples were derivated with 2,4-dinitrofluorobenzen. The buffer solution used in this method was 0.03mol/L sodium borate solution (pH9.8). Column temperature was 17℃. The separating voltage was 28βkV and detection at 360βnm. Factors of pH value, reaction time, reaction temperature and concentration of buffer were optimized. The products of derivation of theanine can be stable for five days at 4℃ or 25℃. The linearity of this assay was 0.2~5βmmol/L (r2=0.993). The minimal detection limit (S/N=2) was 0.05βmmol/L.

Key words: Tea science, theanine, derivative, capillary electrophoresis, determination

CLC Number: