Genetic diversity in 45 Oolong germplasms collected from three provinces, Fujian, Guangdong and Taiwan was evaluated by AFLP fingerprinting based on silver staining technique using 5 primer combinations. The 5 primer combinations generated 208 polymorphic bands. Largest genetic distance between germplasm was 0.481, and the smallest was 0.124. Estimated genetic diversity within the 45 germplasm was up to 0.311. Genetic diversity within Wuyi Mountain population was highest, while genetic diversity within Anxi population was higher than Guangdong and Taiwan populations and lowest within Taiwan population. A dendrogram generated by NT-SYS software indicated that the 45 Oolong tea germplasms could be divided as Wuyi type and Chaoan type. Genetic similarity determined by Popgene 32 software was highest between Anxi and Wuyi populations which was up to 0.9505, and lowest between Taiwan and Chaoan populations which was up to 0.77. It could be inferred that Fujian Oolong tea germplasms originated from Wuyi Mountains.
黄福平,梁月荣,陆建良,陈荣冰,Mamati G. E.,孙庆磊
. Evaluation of Genetic Diversity in Oolong Tea Germplasms by AFLP Fingerprinting[J]. Journal of Tea Science, 2004
, 24(3)
: 183
-189
.
DOI: 10.13305/j.cnki.jts.2004.03.007
[1] 吴敏生, 戴景瑞. 扩增片段长度多态性(AFLP): 一种新的分子标记技术[J]. 植物学通报, 1998, 15(4):68-74.
[2] Paul S, Wachira F.N, Powell W, et al. Diversity and genetic differentiation among populations of India and Kenyan tea (Camellia Sinensis (L.) O. Kuntze) revealed by AFLP markers[J]. Theor Appl.Genet, 1997, 94: 255-263.
[3] Wachira F.N., Waugh R., Hackett C.A, et al. Detection of genetic diversity in tea [camellia sinensis] using RAPD makers[J]. Genome, 1995, Vol.38: 201-210.
[4] Hackett C.A, Wachira F.N, Paul S, et al. Construction of a genetic linkage map for Camellia sinensis(tea)[J]. Heredity, 2000, 346-355.
[5] Wachira F, Tanaka J, Takeda Y. Genetic variation and differentiation in tea (camellia sinensis) germplasm revealed by RAPD and AFLP variation[J]. Journal of Horticultural Science & Biotechnology, 2001, 76(5): 557-563.
[6] Balasaravanan B, Pius P. K, Kumar R. R., et al. Genetic diversity among south Indian tea germplasm(camellia sinensis,C.assamica and C. assamica spp.lasiocalyx)using AFLP markers[J]. Plant Science, 2003, 165: 365-372.
[7] Clark M.S.主编(顾红雅,瞿礼嘉译).植物分子生物学-实验手册。北京:高等教育出版社,1999, 6-7.
[8] Vos P, Hogers R., Bleeker M., et al. AFLP: a new technique for fingerprinting[J].Nucleic Acids research, 1995, Vol.23, No.21: 4407-4414.
[9] Bassam B.J, Anolles G C, Gresshoff P M. Fast and sensitive silver staining of DNA in Polyacrylamide Gels[J]. Analyticl Biochemistry, 1991, 196, 80-83.
[10] Nei M. and Li, W.H..Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proc. Natl. Acad. Sci. USA, 1979, 76: 5269-5273.
[11] Nei M. Analysis of gene diversity in subdivided populations[J]. Proc.Natl.Acad.Sci.USA, 1973, 76: 5269-5273.
[12] Shannon C.E, Weaver W. The mathemmatical theory of communication[M].University of Illinois Press, 1949, Urbana, USA.
[13] Yeh F.C, Yang R.C, Boyle T.B.J, et al. POPGENE,the user friendly shareware for population genetic analysis.Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
[14] 倪郑重, 何融. 乌龙茶工艺史考证[J]. 中国科技史料, 1995, 16(3): 92-96.
[15] 阮逸明. 台湾乌龙茶的发展及特色[J]. 福建茶叶, 2003, 1: 21-23.