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Journal of Tea Science ›› 2004, Vol. 24 ›› Issue (3): 183-189.doi: 10.13305/j.cnki.jts.2004.03.007

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Evaluation of Genetic Diversity in Oolong Tea Germplasms by AFLP Fingerprinting

HUANG Fu-ping1, 2, LIANG Yue-rong1, LU Jian-liang1, CHEN Rong-bing2, Mamati G. E.1, SUN Qing-lei   

  1. 1. Tea Research Institute, Zhejiang University, HangZhou 310029, China;
    2. Tea Research Institute, Fujian Acaedemy of Agricultrual Sciences, Fu’an 355000, China
  • Online:2004-09-25 Published:2019-09-16

Abstract: Genetic diversity in 45 Oolong germplasms collected from three provinces, Fujian, Guangdong and Taiwan was evaluated by AFLP fingerprinting based on silver staining technique using 5 primer combinations. The 5 primer combinations generated 208 polymorphic bands. Largest genetic distance between germplasm was 0.481, and the smallest was 0.124. Estimated genetic diversity within the 45 germplasm was up to 0.311. Genetic diversity within Wuyi Mountain population was highest, while genetic diversity within Anxi population was higher than Guangdong and Taiwan populations and lowest within Taiwan population. A dendrogram generated by NT-SYS software indicated that the 45 Oolong tea germplasms could be divided as Wuyi type and Chaoan type. Genetic similarity determined by Popgene 32 software was highest between Anxi and Wuyi populations which was up to 0.9505, and lowest between Taiwan and Chaoan populations which was up to 0.77. It could be inferred that Fujian Oolong tea germplasms originated from Wuyi Mountains.

Key words: Tea Plant, Camellia sinensis, Oolong tea germplasm, Genetic diversity, AFLP

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