High performance liquid chromatography (HPLC) coupled with evaporative light scattering detector（ELSD-2000）was used for the determination of theanine in tea without derivatization. A Polaris-C18 column (250βmm×4.6βmm) was used with the mobile phase of 0.1%trifluoroacetic acid water solution and 0.1% trifluoroacetic acid acetonitrile solution .The flow rate was at 0.5βml/min. The linear response ranged from 0.062~0.450βmg/mL for theanine. The recovery was 97.5%. The method was evaluated as accurate and simple.
LI Yin-hua
,
LIU Zhong-hua*
,
HUANG Jian-an
,
GONG Yu-shun
. Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography-Evaporative Light-Scattering Detector[J]. Journal of Tea Science, 2005
, 25(3)
: 225
-228
.
DOI: 10.13305/j.cnki.jts.2005.03.012
[1] 肖伟涛, 朱小兰, 陈波, 等. 制备高效液相色谱分离纯化茶氨酸对照品.中草药, 2004, 35(2): 148~150.
[2] 吕毅, 郭雯飞, 倪捷儿, 等. 茶氨酸的生理作用及合成[J]. 茶叶科学, 2003, 23(1):1~5.
[3] Kakuda T, Yanase H.Protective effect of г-glutamylethylamide(theanine) on ischemic delayed neuronal death in gerbils. Neurosci. Lett, 2000, 289(3): 189~192.
[4] Yokogoshi H, Mochizuki M, Saitoh K.Theanine-induced reduction of brain serotonin concentration in rats. Biosci. Biotech. Biochem, 1998, 62(4): 816~817.
[5] Yokogoshi H, Kato Y.Reduction effect of theanine on blood pressure and brain 5-hydroxyindoles I spontaneously hypertensive rats. Biosci. Biotech. Biochem., 1995, 59(4): 615~618.
[6] 朱曙东, 赵皓. 氨基酸的高效液相色谱分析[J]. 色谱, 1994, 12(1): 20~23.
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