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HPLC Analysis, Preparation and Mass Identification of Flavonol Glycosides in Tea Seed Flakes

  • JIANG He-yuan ,
  • KE Chang-qiang ,
  • WANG Chuan-pi ,
  • CHEN Xiao-qiang ,
  • YUAN Xin-yue
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  • 1. Key Laboratory of Tea Chemical Engineering of Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agriculture Sciences, Hangzhou 310008,China;
    2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China

Online published: 2019-09-16

Abstract

Through HPLC and preparing HPLC and the analysis of molecular weight and chemical group by HPLC/MS, Two flavonol glycosides from tea seed flakes were separated and identied. The systematical method combining analytical and preparation scale HPLC with HPLC/MS, could also be used in the study of other natural products in tea.

Cite this article

JIANG He-yuan , KE Chang-qiang , WANG Chuan-pi , CHEN Xiao-qiang , YUAN Xin-yue . HPLC Analysis, Preparation and Mass Identification of Flavonol Glycosides in Tea Seed Flakes[J]. Journal of Tea Science, 2005 , 25(4) : 289 -294 . DOI: 10.13305/j.cnki.jts.2005.04.009

References

[1] 柳荣祥, 朱全芬, 夏春华. 茶皂素表面活性剂及其应用研究进展[J]. 日用化学工业, 1996(5): 32~36.
[2] M Amoros, L Girre.Mechanism of antiviral activity of triterpenoid saponins[J]. Phytotherapy Research, 1999, 13(4): 323~328.
[3] M GUO, F SONG.Rapid Analysis of a Triterpenoid Saponin Mixture from Plant Extracts by Electrospray Ionization Multi-stage Tandem Mass Spectrometry (ESI-MSn)[J]. Analytical Sciences. 2002, 18(4): 481~484.
[4] Ferran S R, Olga J, Isidre C, et al. Liquid chromatographic/ electrospray ionization tandem mass spectrometric study of the phenolic composition of cocoa (Theobroma cacao)[J]. Mass Spectrom., 2003, 38: 35~42.
[5] Hajime S, Masazum M, Susumu I, et al.1993, Saponins from leaves of Acanthopanax Sieboldianus[J]. Phytochemistry, 34(4): 1117~1121.
[6] 贾世山, 马超美, 李英和, 等. 甘草叶中酚酸和黄酮甙类成分的分离鉴定[J]. 药学学报, 1992, 27(6): 441~444.
[7] Masao Y, Hiroharu F, Mihoko M, et al. A chemotaxonomic study of flavonoids in the leaves of six Trichosanthes[J] species. Phytochemistry, 1987, 26(9): 2557~2558.
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