Improvement of White Tea on Cigarette Smoke-induced Chronic Obstructive Pulmonary Disease in Mice

LI Pan; ZHOU Hui; CAI Meisheng; KIM Eunhye; LIU Xiaobo; HE Puming; TU Youying

doi:10.13305/j.cnki.jts.2020.05.010

Journal of Tea Science >

2020 , Vol. 40 >Issue 5: 641 - 655

DOI: https://doi.org/10.13305/j.cnki.jts.2020.05.010

Research Paper

Improvement of White Tea on Cigarette Smoke-induced Chronic Obstructive Pulmonary Disease in Mice

LI Pan ,
ZHOU Hui ,
CAI Meisheng ,
KIM Eunhye ,
LIU Xiaobo ,
HE Puming ,
TU Youying

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1. Department of Tea Science, Zhejiang University, Hangzhou 310058, China;
2. Fuding Tea Industry Development Leading Group, Fuding 355200, China

Received date: 2020-04-15

Revised date: 2020-05-21

Online published: 2020-10-10

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Abstract

Sixty ICR mice were randomly divided into normal control group, model control group, Shoumei group, Baimudan group, Baihaoyinzhen group and epigallocatechin gallate group (EGCG). Chronic obstructive pulmonary (COPD) model of mice was established by smoking method. Three kinds of white tea water extracts and epigallocatechin gallate (EGCG) were administered by intragastric administration and sacrificed after five weeks, and plasma, bronchoalveolar lavage fluid, lung tissue and liver tissue were collected to determine the pathological changes of lung tissue and the biochemical indicators of each group. The improvement effect and mechanism of white tea on COPD in mice were shown as follows: (1) In the model group, a large amount of pathological damage such as inflammatory infiltration and goblet cell metaplasia appeared in the lung tissue. Both white tea extracts and EGCG treatment could obviously improve the pathological damage of the lung tissue, and the Baihaoyinzhen extract had the best effect. (2) Model group showed obvious oxidative stress and inflammation. The levels of malondialdehyde (MDA), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) increased significantly, and the activity of superoxide dismutase (SOD) decreased significantly. Both white tea extracts and EGCG treatment could significantly reduce MDA, IL-6 and TNF-α levels and up-regulate SOD activity. (3) The nitric oxide (NO) level of plasma and myeloperoxidase (MPO) activity of lung tissues increased significantly in model group, on the contrary NO level decreased in BALF and lung tissues. Both the white tea extracts and the EGCG could improve NO disorders and reduced MPO activity. (4) Both white tea extracts and EGCG could improve the decline of phosphorylation level of adenosine monophosphate-dependent protein kinase (AMPK) in COPD mice. No liver toxicity of white tea extracts or EGCG was found in mice under treatments. In summary, white tea extracts could obviously improve cigarette smoke-induced COPD in mice by anti-oxidation, anti-inflammatory and regulating NO abnormalities.

Key words： AMPK; chronic obstructive pulmonary disease; inflammatory factor; oxidative stress; white tea extracts

Cite this article

LI Pan , ZHOU Hui , CAI Meisheng , KIM Eunhye , LIU Xiaobo , HE Puming , TU Youying . Improvement of White Tea on Cigarette Smoke-induced Chronic Obstructive Pulmonary Disease in Mice[J]. Journal of Tea Science, 2020 , 40(5) : 641 -655 . DOI: 10.13305/j.cnki.jts.2020.05.010

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