Improvement of White Tea on Cigarette Smoke-induced Chronic Obstructive Pulmonary Disease in Mice

LI Pan; ZHOU Hui; CAI Meisheng; KIM Eunhye; LIU Xiaobo; HE Puming; TU Youying

doi:10.13305/j.cnki.jts.2020.05.010

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (5) : 641-655. DOI: 10.13305/j.cnki.jts.2020.05.010

Research Paper

Improvement of White Tea on Cigarette Smoke-induced Chronic Obstructive Pulmonary Disease in Mice

LI Pan¹, ZHOU Hui¹, CAI Meisheng², KIM Eunhye¹, LIU Xiaobo¹, HE Puming^1,*, TU Youying^1,*

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Abstract

Sixty ICR mice were randomly divided into normal control group, model control group, Shoumei group, Baimudan group, Baihaoyinzhen group and epigallocatechin gallate group (EGCG). Chronic obstructive pulmonary (COPD) model of mice was established by smoking method. Three kinds of white tea water extracts and epigallocatechin gallate (EGCG) were administered by intragastric administration and sacrificed after five weeks, and plasma, bronchoalveolar lavage fluid, lung tissue and liver tissue were collected to determine the pathological changes of lung tissue and the biochemical indicators of each group. The improvement effect and mechanism of white tea on COPD in mice were shown as follows: (1) In the model group, a large amount of pathological damage such as inflammatory infiltration and goblet cell metaplasia appeared in the lung tissue. Both white tea extracts and EGCG treatment could obviously improve the pathological damage of the lung tissue, and the Baihaoyinzhen extract had the best effect. (2) Model group showed obvious oxidative stress and inflammation. The levels of malondialdehyde (MDA), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) increased significantly, and the activity of superoxide dismutase (SOD) decreased significantly. Both white tea extracts and EGCG treatment could significantly reduce MDA, IL-6 and TNF-α levels and up-regulate SOD activity. (3) The nitric oxide (NO) level of plasma and myeloperoxidase (MPO) activity of lung tissues increased significantly in model group, on the contrary NO level decreased in BALF and lung tissues. Both the white tea extracts and the EGCG could improve NO disorders and reduced MPO activity. (4) Both white tea extracts and EGCG could improve the decline of phosphorylation level of adenosine monophosphate-dependent protein kinase (AMPK) in COPD mice. No liver toxicity of white tea extracts or EGCG was found in mice under treatments. In summary, white tea extracts could obviously improve cigarette smoke-induced COPD in mice by anti-oxidation, anti-inflammatory and regulating NO abnormalities.

Key words

AMPK / chronic obstructive pulmonary disease / inflammatory factor / oxidative stress / white tea extracts

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LI Pan, ZHOU Hui, CAI Meisheng, KIM Eunhye, LIU Xiaobo, HE Puming, TU Youying. Improvement of White Tea on Cigarette Smoke-induced Chronic Obstructive Pulmonary Disease in Mice[J]. Journal of Tea Science. 2020, 40(5): 641-655 https://doi.org/10.13305/j.cnki.jts.2020.05.010

References

[1] Mannino D M, Kiri V A.Changing the burden of COPD mortality[J]. International Journal of COPD, 2013, 1(3): 219-233.
[2] Burney P G J, Patel J, Newson R, et al. Global and regional trends in COPD mortality, 1990-2010[J]. European Respiratory Journal, 2015, 45(5): 1239-1247.
[3] Vogelmeier C F, Criner G J, Martinez F J, et al.Global strategy for the diagnosis, management, and prevention of chronic obstructive lung disease 2017 report: GOLD executive summary[J]. American Journal of Respiratory and Critical Care Medicine, 2017, 195(5): 557-582.
[4] Postma D S, Bush A, Maarten V D B. Risk factors and early origins of chronic obstructive pulmonary disease[J]. The Lancet, 2015, 385(9971): 899-909.
[5] Goel R, Bitzer Z, Reilly S M, et al.Variation in free radical yields from US marketed cigarettes[J]. Chemical Research in Toxicology, 2017, 30(4): 1038-1045.
[6] Barnes P J, Shapiro S D, Pauwels R A.Chronic obstructive pulmonary disease: molecular and cellular mechanisms[J]. European Respiratory Journal, 2003, 22(4): 672-688.
[7] Calverley P.Current drug treatment, chronic and acute[J]. Clinics in Chest Medicine, 2014, 35(1): 177-189.
[8] Barnes, P J.Inhaled corticosteroids in COPD: a controversy[J]. Respiration, 2010, 80(2): 89-95.
[9] Boardman C, Chachi L, Gavrila A, et al.Mechanisms of glucocorticoid action and insensitivity in airways disease[J]. Pulmonary Pharmacology & Therapeutics, 2014, 29(2): 129-143.
[10] Kawamatawong T.Roles of roflumilast, a selective phosphodiesterase 4 inhibitor, in airway diseases[J]. Journal of Thoracic Disease, 2017, 9(4): 1144-1154.
[11] Gonçalves P B, Romeiro N C.Multi-target natural products as alternatives against oxidative stress in COPD[J]. European Journal of Medicinal Chemistry, 2019, 163: 911-931.
[12] Cheng T O.All teas are not created equal: the Chinese green tea and cardiovascular health[J]. International Journal of Cardiology, 2006, 108(3): 301-308.
[13] Yang C S, Hong J.Prevention of chronic diseases by tea: possible mechanisms and human relevance[J]. Annual Review of Nutrition, 2013, 33(1): 161-181.
[14] Chan K H, Chan S C H, Yeung S C, et al. Inhibitory effect of Chinese green tea on cigarette smoke-induced up-regulation of airway neutrophil elastase and matrix metalloproteinase-12 via antioxidant activity[J]. Free Radical Research, 2012, 46(9): 1123-1129.
[15] Yingmin L, Liu K W K, Yeung S C, et al. (-)-epigallocatechin-3-gallate reduces cigarette smoke-induced airway neutrophilic inflammation and mucin hypersecretion in rats[J]. Frontiers in Pharmacology, 2017, 8: 618. doi: 10.3389/fphar.2017.00618.
[16] 黄翔翔, 杨哲, 禹利君. 绿茶、EGCG预防及缓解香烟烟雾诱导COPD的研究进展[J]. 茶叶科学, 2017, 37(4): 332-338.
Huang X X, Yang Z, Yu L J.Research Progress of Green Tea and EGCG for the prevention and mitigation of chronic obstructive pulmonary disease caused by cigarette smoke[J]. Journal of Tea Science, 2017, 37(4): 332-338.
[17] 中华人民共和国卫生部. 保健食品检验与评价技术规范(2003年版)[S]. 北京: 卫生部卫生法制与监督司编印, 2003: 115-117.
Ministry of Health of the People's Republic of China. Technical specifications for health food inspection and evaluation (2003 edition)[S]. Beijing: Published by the Department of Health Legal System and Supervision, Ministry of Health, 2003: 115-117.
[18] 张正竹. 茶叶生物化学实验教程 [M]. 第一版. 北京: 中国农业出版社, 2009: 35-39, 42-45.
Zhang Z Z.Tea Biochemistry Experiment Course [M]. 1st ed. Beijing: China Agriculture Press, 2009: 35-39, 42-45.
[19] 张国权, 罗崧方, 黄建蓉, 等. 三氯化铝比色法检测桑叶提取物总黄酮的研究[J]. 农产品加工, 2018(4): 52-54,57.
Zhang G Q, Luo S F, Huang J R, et al.Research on determination of total flavonoids in mulberry leaf extract by aluminum chloride colorimetry[J]. Agricultural Products Processing, 2018(4): 52-54,57.
[20] 曲春香, 沈颂东, 王雪峰, 等. 用考马斯亮蓝测定植物粗提液中可溶性蛋白质含量方法的研究[J]. 苏州大学学报(自然科学版), 2006, 22(2): 82-85.
Qu C X, Shen S D, Wang X F, et al.Method research of measuring soluble protein contents of plant rough extraction using Coomassie Brilliant Blue[J]. Journal of Suzhou University (Natural Science Edition), 2006, 22(2): 82-85.
[21] 中国国家标准化管理委员会. 茶叶中茶多酚和儿茶素类含量的检测方法: GB/T 8313—2018 [S]. 北京: 中国标准出版社, 2018.
China National Standardization Management Committee. Detection method of tea polyphenols and catechins in tea: GB/T 8313—2018 [S]. Beijing: China Standard Press, 2018.
[22] 王蝶. 茶叶对肥胖大鼠的减肥作用及机制研究[D]. 长沙: 湖南农业大学, 2012: 10-11.
Wang D.Research on the weight loss effect and mechanism of tea on obese rats [D]. Changsha: Hunan Agricultural University, 2012: 10-11.
[23] Chan P C, Ramot Y, Malarkey D E, et al.Fourteen-week toxicity study of green tea extract in rats and mice[J]. Toxicologic Pathology, 2010, 38(7): 1070-1084.
[24] Luo F, Liu J, Yan T, et al.Salidroside alleviates cigarette smoke-induced COPD in mice[J]. Biomedicine & Pharmacotherapy, 2017, 86: 155-161.
[25] 王秦川, 陈宇, 汤慧芳, 等. ICR小鼠吸烟诱导肺部炎症模型的建立及其特征[J]. 浙江大学学报(医学版), 2008, 37(4): 328-332.
Wang Q C, Chen Y, Tang H F, et al.Establishment and characteristics of smoking-induced lung inflammation model in ICR mice[J]. Journal of Zhejiang University (Medical Sciences), 2008, 37(4): 328-332.
[26] 张科东, 马冉, 李征途, 等. 一种熏烟小鼠慢性阻塞性肺疾病模型的建立及鉴定[J]. 国际呼吸杂志, 2012, 32(21): 1607-1611.
Zhang K D, Ma R, Li Z T, et al.Establishment and identification of a chronic obstructive pulmonary disease model in smoked mice[J]. International Journal of Respiration, 2012, 32(21): 1607-1611.
[27] Yu D, Liu X, Zhang G X, et al.Isoliquiritigenin inhibits cigarette smoke-induced COPD by attenuating inflammation and oxidative stress via the regulation of the Nrf2 and NF-κB signaling pathways[J]. Frontiers in Pharmacology, 2018, 9: 1001. doi: 10.3389/fphar.2018.01001.
[28] Ichinose M, Sugiura H, Yamagata S, et al.Increase in reactive nitrogen species production in chronic obstructive pulmonary disease airways[J]. American Journal of Respiratory and Critical Care Medicine, 2000, 162(2): 701-706.
[29] Wang X, Lu G, Picinich S C, et al.Cancer prevention by tea: animal studies, molecular mechanisms and human relevance[J]. Nature Reviews Cancer, 2009, 9(6): 429-439.
[30] Thring T S, Hili P, Naughton D P.Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants[J]. BMC Complementary and Alternative Medicine, 2009, 9: 27. doi: 10.1186/1472-6882-9-27.
[31] Zuo L, He F, Sergakis G G, et al.Interrelated role of cigarette smoking, oxidative stress, and immune response in COPD and corresponding treatments[J]. AJP Lung Cellular and Molecular Physiology, 2014, 307(3): 205-218.
[32] Barnes P J.The cytokine network in chronic obstructive pulmonary disease[J]. American Journal of Respiratory Cell and Molecular Biology, 2009, 41(6): 631-638.
[33] Aaron S D, Angel J B, Lunau M, et al.Granulocyte inflammatory markers and airway infectionduring acute exacerbation of chronic obstructive pulmonary disease[J]. American Journal of Respiratory and Critical Care Medicine, 2001, 163(2): 349-355.
[34] Bhowmik A, Seemungal T A R, Sapsford R J, et al. Relation of sputum inflammatory markers to symptoms and lung function changes in COPD exacerbations[J]. Thorax, 2000, 55: 114-120.
[35] Barnes P J, Celli B R.Systemic manifestations and comorbidities of COPD[J]. European Respiratory Journal, 2009, 33(5): 1165-1185.
[36] 史春麟, 李晓焕, 黄翔翔. 绿茶多酚对被动吸烟引起小鼠肺氧化应激的干预研究[J]. 茶叶科学, 2018, 38(2): 212-220.
Shi C L, Li X H, Huang Y Y.Intervention research of green tea polyphenols on oxidative stress in mice lungs induced by passive smoking[J]. Journal of Tea Science, 2018, 38(2): 212-220.
[37] 周进, 黄文涛, 代能捷, 等. EGCG调控miR-133a/b-3p和TGF-β1/Smad3抗慢性阻塞性肺疾病的作用研究[J]. 中药材, 2018, 41(2): 437-441.
Zhou J, Huang W T, Dai N J, et al.Study on the anti-chronic obstructive pulmonary disease effect of EGCG regulating miR-133a/b-3p and TGF-β1/Smad3[J]. Journal of Chinese Medicinal Materials, 2018, 41(2): 437-441.
[38] Gross S S, Wolin M S.Nitric oxide: pathophysiological mechanisms[J]. Annual Review of Physiology, 1995, 57(1): 737-769.
[39] Montuschi P, Kharitonov S A, Barnes P J.Exhaled carbon monoxide and nitric oxide in COPD[J]. Chest, 2001, 120(2): 496-501.
[40] Brindicci C, Ito K, Resta O, et al.Exhaled nitric oxide from lung periphery is increased in COPD[J]. European Respiratory Journal, 2005, 26(1): 52-59.
[41] Maziak W, Loukides S, Culpitt S, et al.Exhaled nitric oxide in chronic obstructive pulmonary disease[J]. American Journal of Respiratory and Critical Care Medicine, 1998, 157(3): 998-1002.
[42] Shaul P W, Smart E J, Robinson L J, et al.Acylation targets endothelial nitric-oxide synthase to plasmalemmal caveolae[J]. Journal of Biological Chemistry, 1996, 271(11): 6518-6522.
[43] Cho H J, Xie Q W, Calaycay J, et al.Calmodulin as a tightly bound subunit of calcium/calmodulin-independent nitric oxide synthase[J]. Journal of Experimental Medicine, 1992, 176: 599-604.
[44] Stuehr D J.Structure-function aspects in the nitric oxide synthases[J]. Annual Review of Pharmacology and Toxicology, 1997, 37(1): 339-359.
[45] Shaul P W, North A J, Brannon T S, et al.Prolonged in vivo hypoxia enhances nitric oxide synthase type Ⅰ and type Ⅲ gene expression in adult rat lung[J]. American Journal of Respiratory Cell and Molecular Biology, 1995, 13(2): 167-174.
[46] Abdelghany T M, Ismail R S, Mansoor F A, et al.Cigarette smoke constituents cause endothelial nitric oxide synthase dysfunction and uncoupling due to depletion of tetrahydrobiopterin with degradation of GTP cyclohydrolase[J]. Nitric Oxide, 2018, 76: 113-121.
[47] Eiserich J P, Hristova M, Cross C E, et al.Formation of nitric oxide-derived inflammatory oxidants by myeloperoxidase in neutrophils[J]. Nature, 1998, 391(6665): 393-397.
[48] Yang C S, Zhang J, Zhang L, et al.Mechanisms of body weight reduction and metabolic syndrome alleviation by tea[J]. Molecular Nutrition & Food Research, 2016, 60(1): 160-174.
[49] Lee J S, Park S J, Cho Y S, et al.Role of AMP-activated protein kinase (AMPK) in smoking-induced lung inflammation and emphysema[J]. Tuberculosis and Respiratory Diseases, 2015, 78(1): 8-17.
[50] Cheng X Y, Li Y Y, Huang C, et al.AMP-activated protein kinase reduces inflammatory responses and cellular senescence in pulmonary emphysema[J]. Oncotarget, 2017, 8(14): 22513-22523.
[51] Lee K Y, Kim J R, Choi H C.Genistein-induced LKB1-AMPK activation inhibits senescence of VSMC through autophagy induction[J]. Vascular Pharmacology, 2016, 81: 75-82.
[52] Zhang Z, Cheng X, Yue L, et al.Molecular pathogenesis in chronic obstructive pulmonary disease and therapeutic potential by targeting AMP-activated protein kinase[J]. Journal of Cellular Physiology, 2018, 233(3): 1999-2006.
[53] Yamashita Y, Wang L, Wang L, et al.Oolong, black and puer tea suppresses adiposity in mice via activation of AMP-activated protein kinase[J]. Food & Function, 2014, 5(10): 2420-2429.
[54] Murase T, Misawa K, Haramizu S, et al.Catechin-induced activation of the LKB1/AMP-activated protein kinase pathway[J]. Biochemical Pharmacology, 2009, 78(1): 78-84.
[55] Collins Q F, Liu H Y, Pi J, et al.Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, suppresses hepatic gluconeogenesis through 5'-AMP-activated protein kinase[J]. Journal of Biological Chemistry, 2007, 282(41): 30143-30149.
[56] Mazzanti G, Menniti-Ippolito F, Moro P A, et al.Hepatotoxicity from green tea: a review of the literature and two unpublished cases[J]. European Journal of Clinical Pharmacology, 2009, 65(4): 331-341.
[57] Schönthal A H.Adverse effects of concentrated green tea extracts[J]. Molecular Nutrition & Food Research, 2011, 55(6): 874-885.
[58] Lambert J D, Kennett M J, Sang S, et al.Hepatotoxicity of high oral dose (-)-epigallocatechin-3-gallate in mice[J]. Food and chemical toxicology, 2010, 48(1): 409-416.
[59] 曾琪, 任发政, 雷新根, 等. 白茶体外抗氧化与体内抗衰老作用的研究[J]. 茶叶科学, 2018, 38(6): 615-624.
Zeng Q, Ren F Z, Lei X G, et al.Research on white tea's anti-oxidation in vitro and anti-aging in vivo[J]. Journal of Tea Science, 2018, 38(6): 615-624.