Identification of Pectin Methylesterase and Its Inhibitory Subfamily Genes, and Functional Analysis of CsPME55 in Response to Fluoride Stress in Camellia sinensis

XU Wenluan; WEN Xiaoju; JIA Yuxuan; NI Dejiang; WANG Mingle; CHEN Yuqiong

doi:10.13305/j.cnki.jts.2024.06.008

2024 , Vol. 44 >Issue 6: 869 - 886

DOI: https://doi.org/10.13305/j.cnki.jts.2024.06.008

Research Paper

Identification of Pectin Methylesterase and Its Inhibitory Subfamily Genes, and Functional Analysis of CsPME55 in Response to Fluoride Stress in Camellia sinensis

XU Wenluan ,
WEN Xiaoju ,
JIA Yuxuan ,
NI Dejiang ,
WANG Mingle ,
CHEN Yuqiong

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National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2024-10-29

Revised date: 2024-11-20

Online published: 2025-01-08

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Abstract

Tea plant (Camellia sinensis) has fluoride (F) enrichment characteristics, and F is mainly enriched in the cell wall component pectin. Pectin methylsterase (PME) and its inhibitor PMEI can catalyze the modification of pectin, thereby affecting cell wall characteristics and participating in the regulation of processes like plant growth and development, stress response and so on. In this study, 85 CsPMEs and 56 CsPMEIs were identified from the C. sinensis ‘Shuchazao’ genome, which were divided into 4 and 5 subgroups, respectively. Distinct subgroups may exhibit functional distinction due to varied gene architectures, conserved motifs and expression patterns. Quantitative real-time PCR (qRT-PCR) analysis reveals that the expression levels of CsPME3a, CsPME55, CsPMEI1 and CsPMEI3 were significantly induced in the mature leaves of ‘Fuding Dabaicha’ under F treatment. Moreover, overexpression of CsPME55 alleviated Arabidopsis root growth inhibition induced by F stress, suggesting its potential role in F stress regulation in tea plants. These findings could pave the way for further research on the functional involvement of PME and PMEI gene families in F response.

Key words： Camellia sinensis; fluoride; functional analysis; gene expression; pectin methylesterase; pectin methylesterase inhibitor

Cite this article

XU Wenluan , WEN Xiaoju , JIA Yuxuan , NI Dejiang , WANG Mingle , CHEN Yuqiong . Identification of Pectin Methylesterase and Its Inhibitory Subfamily Genes, and Functional Analysis of CsPME55 in Response to Fluoride Stress in Camellia sinensis[J]. Journal of Tea Science, 2024 , 44(6) : 869 -886 . DOI: 10.13305/j.cnki.jts.2024.06.008

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