In order to explore the absorption regularity of TF1 and EGCG in the organism. This research used the Caco-2 cell monolayer model in vitro to simulate the absorption of TF1 and EGCG in the small intestine. The influences of concentration and time on the absorption regularity of TF1 and EGCG in Caco-2 cell monolayer model were investigated. The results illustrated that the absorption of TF1 and EGCG in Caco-2 model showed that the apparent permeability coefficient raised with the increasing of concentrations of two compound in the range of 10~100βµmol·L-1. The efflux rates of the TF1 and EGCG showed the same rules as absorption. However, the increasing range of efflux rate was higher than that of absorption rate. The values of Papp about TF1 and EGCG in the cell model were lower than 1×10-6βcm·s -1, which indicated that both of them belonged to the kind of drugs which were difficult to absorb. However, with comparing the absorption rate, TF1 was higher than EGCG in this model. Both of efflux transport showed the passive process because the efflux rates of TF1 and EGCG were larger than 2 and higher than in cell model. Because the efflux regulation of TF1 was in accordance with EGCG by temporal variation, it suggested that both of them were the substrate of the same efflux protein.
MENG Qing
,
TU Youying
. Study on Absorption of Theaflavin and EGCG in Caco-2 Cell Model[J]. Journal of Tea Science, 2015
, 35(3)
: 233
-238
.
DOI: 10.13305/j.cnki.jts.2015.03.005
[1] 屠幼英. 茶与健康[M]. 西安: 世界图书出版西安有限公司, 2011: 16-22.
[2] Wu Y Y, Li W, Xu Y, et al. Evaluation of the antioxidant effects of four main theaflavin derivatives through chemiluminescence and DNA damage analyses[J]. Journal of Zhejiang University-Science B, 2011, 12(9): 744-751.
[3] Tu Y Y, Tang A B, N Watanabe. The Theaflavin Monomers Inhibit the Cancer Cells Growth in Vitro[J]. Acta Biochimica et Biophysica Sinica, 2004, 36(7): 508-512.
[4] Li W, Wu J X, Tu Y Y.Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells[J]. Journal of Zhejiang University-Science B, 2010, 11(6):458-464.
[5] 徐懿. 茶黄素的分离制备及其降脂减肥研究[D]. 杭州: 浙江大学, 2011: 19-28.
[6] Artursson P, Borchardt R T.Intestinal drug absorption and metabolism in cell cultures: Caco-2 and beyond[J]. Pharmaceutical Research, 1997, 14(12): 1655-1658.
[7] Zhang L, Zheng Y, Chow M S S, et al. Investigation of intestinal absorption and disposition of green tea catechins by Caco-2 monolayer model[J]. International Journal of Pharmaceutics, 2004, 287: 1-12.
[8] Neilson A P, Song B J, Sapper T N, et al. Tea catechin auto-oxidation dimers are accumulated and retained by Caco-2 human intestinal cells[J]. Nutrition Research, 2010, 30(5): 327-340.
[9] Artursson P, Palm K, Luthman K.Caco-2 monolayers in experimental and theoretical predictions of drug transport[J]. Advanced Drug Delivery Reviews, 1996, 22(1/2): 67-84.
[10] 郭子涛. 表没食子儿茶素没食子酸酯(EGCG)在Caco-2细胞中的摄取、跨膜转运和外排研究[D]. 上海: 华东师范大学, 2011: 24-31.
[11] 屠幼英, 杨子银, 东方. 红茶中多酚类物质的抗氧化机制及其构效关系[J]. 中草药, 2007, 38(10): 1581-1585.
[12] Su Y L, Leung L K, Huang Y, et al. Stability of tea theaflavins and catechins[J]. Food Chemistry, 2003, 83(2): 189-195.
[13] Chan K Y, Zhang L, Zuo Z.Intestinal efflux transport kinetics of green tea catechins in Caco-2 monolayer model[J]. Journal of Pharmacy and Pharmacology, 2007, 59(3): 395-400.
[14] Yang C S, Wang X, Lu G, et al. Cancer prevention by tea: animal studies, molecular mechanisms and human relevance[J]. Nature Reviews Cancer, 2009, 9(6): 429-439.
浙公网安备 33019902000101号