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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (3): 233-238.doi: 10.13305/j.cnki.jts.2015.03.005

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Study on Absorption of Theaflavin and EGCG in Caco-2 Cell Model

MENG Qing, TU Youying*   

  1. Department of tea science, Zhejiang University, Hangzhou 310058, China
  • Received:2015-01-14 Revised:2015-04-13 Online:2015-06-15 Published:2019-08-23

Abstract: In order to explore the absorption regularity of TF1 and EGCG in the organism. This research used the Caco-2 cell monolayer model in vitro to simulate the absorption of TF1 and EGCG in the small intestine. The influences of concentration and time on the absorption regularity of TF1 and EGCG in Caco-2 cell monolayer model were investigated. The results illustrated that the absorption of TF1 and EGCG in Caco-2 model showed that the apparent permeability coefficient raised with the increasing of concentrations of two compound in the range of 10~100βµmol·L-1. The efflux rates of the TF1 and EGCG showed the same rules as absorption. However, the increasing range of efflux rate was higher than that of absorption rate. The values of Papp about TF1 and EGCG in the cell model were lower than 1×10-6βcm·s -1, which indicated that both of them belonged to the kind of drugs which were difficult to absorb. However, with comparing the absorption rate, TF1 was higher than EGCG in this model. Both of efflux transport showed the passive process because the efflux rates of TF1 and EGCG were larger than 2 and higher than in cell model. Because the efflux regulation of TF1 was in accordance with EGCG by temporal variation, it suggested that both of them were the substrate of the same efflux protein.

Key words: TF1, EGCG, Caco-2 model, absorption regularity

CLC Number: