SPF grade Balb/c female mice were used for this experiment. After adaptive feeding for 3 d, the mice were pre-treated orally with different dosages of L-theanine once daily for 30 d. ETEC E44813 intraperitoneal injection was subsequently performed and samples were collected 5h later. The coefficient of liver and spleen, levels of serum alanine aminotranferease (ALT), aspartate aminotransferase (AST) as well as liver homogenate malondialdehyde (MDA), and activities of liver homogenate superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), pathological changes of liver, expressions of serum γ-Interferon (IFN-γ) and Interleukin-4 (IL-4) were studied to explore the protective effects of L-theanine on liver of ETEC immune mouse model. Results show that pretreatment with L-theanine significantly reduced the increase of liver and spleen coefficient caused by ETEC infection|lowered the levels of serum ALT|AST and liver homogenate MDA|increased the activities of liver homogenate SOD|CAT|GSH-Px|decreased the expressions of serum IFN-γ|IL-4|and improved pathological injury of liver tissue. Therefore|it can be concluded that L-theanine is able to prevent ETEC-induced hepatic injury through depressing IFN-γ, IL-4 and inflammatory reaction and enhancing hepatocyte antioxidant abilities.
LIU Qiuling
,
GONG Zhihua
,
CHENG Ling
,
LIU Zunying
,
DENG Yanli
,
CHEN Dong
,
XIAO Wenjun
. Study on the Protective Effect of L-Theanine on the Liver of Mice with Immune Stress Induced by ETEC[J]. Journal of Tea Science, 2016
, 36(5)
: 484
-490
.
DOI: 10.13305/j.cnki.jts.2016.05.006
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