从草履蚧僵虫体内分离出12株病原真菌,对蓟马类昆虫具有70%~90%的致死率。现选出一株病原真菌CYT10利用形态和分子的特点进行鉴定。扫描电镜下观察,该菌产孢细胞簇生瓶状,“之”型产孢轴。经PCR获得rDNA-ITS和EF1-a核苷酸序列,与其他球孢白僵菌的相应序列一致性达95%以上,进化分析CYT10菌株与球孢白僵菌Beauveria bassiana聚为一类。根据菌株的形态观察和DNA序列分析确定该菌为球孢白僵菌。
12 different entomopathogenic fungi isolated from diseased Drosicha corpulenta Kuwana showed high virulence with 70%~90% mortality. CYT10 strain was identified based on the morphological and molecular characteristics. Conidiogenous cells were short-globose to flask-shaped and the conidiophore elongated in a long zig-zag extension after each conidium is produced. The result of Blast search indicated that the ITS and EF1-α sequence of CYT10 showed a high similarity with those of other strain of Beauveria bassiana (>95%). Phylogenetic analysis showed that it was closely related to ARSEF-1185 strain, which was belonging to B. bassiana. CYT10 strain was classified as a member of the species B. bassiana based on the morphological characteristics and analysis of DNA sequences.
[1] Todorova SI, Cloutier C, Cote JC.Pathogenicity of six isolates of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina, Hyphomycetes) to Perillus cioculatus (F) (Henm, Pentatomidae)[J]. Appl Entomol, 2002, 126(4): 182-185.
[2] Rehner SA, Buckley E.A Beauveria phylogeny inferred from nuclear ITS and EF1-a sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs[J]. Mycologia, 2005, 97(1): 84-98.
[3] 李荣森, 罗绍彬, 张用梅, 等. 微生物防治害虫[M]. 北京: 科学出版社, 1983: 27-45.
[4] 冯明祥, 姜瑞德. 崂山茶区茶树害虫的发生及生态控制方法探讨[J]. 中国茶叶, 2010, 32(2): 10-12.
[5] 谢振伦. 茶黄蓟马的生物学特性与防治[J]. 茶叶科学, 1987, 7(2): 29-34.
[6] 张衍炽. 茶黄蓟马的发生与防治[J]. 茶叶科学与技术, 2008(1): 49.
[7] 姚雍静, 卢天国, 郭灿, 等. 植物浸提液防治茶棍蓟马药效试验[J]. 茶叶科学, 2010, 38(3): 26-28.
[8] 王国华, 夏绍湄, 韩宝瑜. 贵州茶园害虫区系考查及优势种演替趋势分析[J]. 安徽农业大学学报, 2010, 37(3): 411-416.
[9] 王定锋, 吴光远, 王庆森, 等. 茶卷叶蛾寄生真菌的分离、鉴定及毒力测定[J]. 福建农业学报, 2010, 25(3): 345-349.
[10] 殷坤山. 茶树害虫的三种主要病原微生物(续)[J]. 中国茶叶, 2006, 28(5): 28-29.
[11] 方卫国, 杨星勇, 张永军, 等. 真菌核酸的一种快速提取方法[J]. 应用与环境生物学报, 2002, 89(3): 305-307.
[12] 中国科学院微生物研究所. 常见及常用真菌[M]. 北京: 科学出版社, 1978.
[13] Sevim A, Demir I, Demirbağ Z.Molecular characterization and virulence of Beauveria spp.from the pine processionary moth, Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae)[J]. Mycopathologia, 2010a, 170(4): 269-77.
[14] Sevim A, Demir I, Hofte M, et al. Isolation and characterization of entomopathogenic fungi from hazelnut-growing region of Turkey[J]. BioControl, 2010b, 55(2): 279-297.
浙公网安备 33019902000101号 
