The investigation optimum analytical conditions in the determination of theaflavins(TFs) with capillary electrophoresis was described. The electrolyte solution was consisted of 250βmmol/L H3BO4, 10βmmol/L KH2PO4, 0.75βmmol/L SDS and 20% (v/v) acetonitrile under pH7.5. The work parameters of the instrument were selected as follows: voltage 25βkV, column temperature 20℃, and detecting wavelength 200βnm. By using this method, the four majors of TF could be separated each other completely within 10 minutes and each had a good linear relationship between its peak area and corresponding concentration (r=0.9945~0.9990). The minimum concentration limit was between 0.53~0.64βμg/ml. The mean recoveries of TFs monomers varied from 92.50% to 108.36%. The coefficients of variation were less than 2.67%. Results showed that the method was simple, rapid and accurate for TFs to be determined in black tea and TFs products.
XIA Wen-juan
,
ZHANG Li-xia
,
SHI Zuo-an
,
JIA Ming
. Analysis of Theaflavins by Capillary Electrophoresis[J]. Journal of Tea Science, 2006
, 26(2)
: 147
-153
.
DOI: 10.13305/j.cnki.jts.2006.02.014
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