Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2006, Vol. 26 ›› Issue (2): 147-153.doi: 10.13305/j.cnki.jts.2006.02.014

Previous Articles     Next Articles

Analysis of Theaflavins by Capillary Electrophoresis

XIA Wen-juan, ZHANG Li-xia*, SHI Zuo-an, JIA Ming   

  1. College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, China
  • Received:2005-09-26 Revised:2006-03-01 Online:2006-06-25 Published:2019-09-10

Abstract: The investigation optimum analytical conditions in the determination of theaflavins(TFs) with capillary electrophoresis was described. The electrolyte solution was consisted of 250βmmol/L H3BO4, 10βmmol/L KH2PO4, 0.75βmmol/L SDS and 20% (v/v) acetonitrile under pH7.5. The work parameters of the instrument were selected as follows: voltage 25βkV, column temperature 20℃, and detecting wavelength 200βnm. By using this method, the four majors of TF could be separated each other completely within 10 minutes and each had a good linear relationship between its peak area and corresponding concentration (r=0.9945~0.9990). The minimum concentration limit was between 0.53~0.64βμg/ml. The mean recoveries of TFs monomers varied from 92.50% to 108.36%. The coefficients of variation were less than 2.67%. Results showed that the method was simple, rapid and accurate for TFs to be determined in black tea and TFs products.

Key words: theaflavins, analytic method, capillary electrophoresis

CLC Number: