The investigation optimum analytical conditions in the determination of theaflavins(TFs) with capillary electrophoresis was described. The electrolyte solution was consisted of 250βmmol/L H3BO4, 10βmmol/L KH2PO4, 0.75βmmol/L SDS and 20% (v/v) acetonitrile under pH7.5. The work parameters of the instrument were selected as follows: voltage 25βkV, column temperature 20℃, and detecting wavelength 200βnm. By using this method, the four majors of TF could be separated each other completely within 10 minutes and each had a good linear relationship between its peak area and corresponding concentration (r=0.9945~0.9990). The minimum concentration limit was between 0.53~0.64βμg/ml. The mean recoveries of TFs monomers varied from 92.50% to 108.36%. The coefficients of variation were less than 2.67%. Results showed that the method was simple, rapid and accurate for TFs to be determined in black tea and TFs products.
Key words
analytic method /
capillary electrophoresis /
theaflavins
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References
[1] P D Collier, R. Mallows.Estimation of theaflavins in tea by gas—liquid chromatography of their trimethylsilyl ethers[J]. Journal of Chromatography A, 1971(157): 19~27.
[2] W Łuczaj, E, Skrzydlewska.Antioxidant properties of black tea in alcohol intoxication[J]. Food and Chemical Toxicology, 2004, 42(12): 2045~2051.
[3] 王坤波, 刘仲华, 黄建安. 茶黄素分析方法研究进展[J]. 食品科学, 2001, 22(11): 104~106.
[4] 王坤波, 刘仲华, 黄建安, 等. 高效液相色谱法测定红茶中的茶黄素[J]. 色谱, 2004, 22(2): 151~153.
[5] 罗国安, 王义明. 毛细管电泳的原理及应用[J]. 色谱, 1995, 13(4): 254~256.
[6] Bee-Lan Lee, Choon-Nam Ong.Comparative analysis of tea catachins and theaflavins by high-performance liquid chromatography and capillary electrophoresis[J]. Journal of Chromatography A, 2000(881): 439~447.
[7] Louwrance Peter Wright, Jean Pieter Aucamp, Zeno Apostolides.Analysis of black tea theaflavins by non-aqueous capillary electrophoresis[J]. Journal of Chromatography A, 2001(919): 205~213.
[8] 梁月荣. 红茶茶黄素类化合物的高效液相色谱-光电二极管阵列检测[J]. 浙江农业大学学报, 1992, 18(S): 70~74.
[9] 杨贤强, 王岳飞, 陈留记. 茶多酚化学(第1版)[M]. 上海: 上海科学技术出版社, 2003.
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