绿茶多酚对被动吸烟引起小鼠肺氧化应激的干预研究

doi:10.13305/j.cnki.jts.2018.02.012

茶叶科学 ›› 2018, Vol. 38 ›› Issue (2): 212-220.doi: 10.13305/j.cnki.jts.2018.02.012

• • 上一篇

绿茶多酚对被动吸烟引起小鼠肺氧化应激的干预研究

史春麟¹, 李晓焕², 黄翔翔^2,*

1. 湖南师范大学附属中学,湖南长沙 410006;
2. 湖南农业大学茶学教育部重点实验室,湖南长沙 410128

收稿日期:2017-09-07 修回日期:2017-10-30 出版日期:2018-04-15 发布日期:2019-08-28
通讯作者: *huangxiangxiangsg@qq.com
作者简介:史春麟,男,湖南师范大学附属中学2016级学生。
基金资助:
湖南师范大学附属中学自主研究性学习项目、湖南省植物功能成分利用协同创新项目（HNCR-2014003）

Effects of Green Tea Polyphenols on Oxidative Stress Induced by Passive Smoking in Mice Lung

SHI Chunlin¹, LI Xiaohuan², HUANG Xiangxiang^2,*

1. The High School Attached to Hunan Normal University, Changsha 410006, China;
2. Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China

Received:2017-09-07 Revised:2017-10-30 Online:2018-04-15 Published:2019-08-28

摘要/Abstract

摘要： 研究了绿茶多酚（Green tea polyphenol,GTP）对被动吸烟致小鼠肺部氧化应激损伤的干预作用,并探讨其可能机制。将40只KM雌性小鼠随机分成正常对照C组、被动吸烟模型M组、100βmg·kg^-1 GTP1组、200βmg·kg^-1 GTP2组,每组10只。实验12周结束后处死小鼠,测定其肺质量及血清氧化应激炎症水平;采用荧光定量PCR测定白细胞介素6（IL-6）、白细胞介素33（IL-33）、肿瘤坏死因子-α（TNF-α）和白细胞介素1β（IL-1β）基因表达水平。研究结果表明,与M组相比,灌喂GTP后使得小鼠生存质量、肺形态有明显改观,显著提高小鼠血清T-SOD及GSH-Px活力,显著降低MDA、IL-6、TNF-α表达水平,显著抑制IL-6、IL-33、TNF-α及IL-1β炎性相关基因的表达,灌胃200βmg·kg^-1 GTP比100βmg·kg^-1的GTP作用更加显著。研究发现,GTP可能通过抑制炎性细胞因子表达水平、提高抗氧化能力来保护肺部组织形态与结构的完整,保护被动吸烟对肺部的损害。

关键词: 绿茶多酚, 被动吸烟, 肺组织, 氧化应激

Abstract: The objective of the study is to investigate the intervention effects of green tea polyphenols (GTP) on oxidative stress induced by passive smoking in mice lung and its possible mechanisms. Forty healthy Kunming mice (female) were randomly divided into 4 groups including normal control group (C), passive smoking model group (M), GTP treated group 1 (GTP1, 100βmg·kg^-1) and GTP treated group 2 (GTP2, 200βmg·kg^-1) respectively (n=10). All mice were killed after treating with GTP for 12 weeks. The morphology and quality of lung, the oxidative stress of serum and inflammatory level were determined. The mRNA expression levels of inflammation-related genes including interleukin 6 (IL-6), interleukin 33 (IL-33), tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β) were measured by fluorescence quantitative PCR. Compared to M group, the life quality and pulmonary morphology of mice in GTP1 and GTP2 were significantly improved, and T-SOD and GSH-Px activities in serum were also significantly enhanced. But MDA, IL-6 and TNF-α levels in serum were decreased significantly. The mRNA expressions of IL-6, IL-33, TNF-α and IL-1β were also declined in GTP groups, which were clearly dose-dependent. These results showed that GTP could protect lung against damage of passive smoking and remain the integrity of lung tissues by inhibiting inflammatory cytokine expression and improving the antioxidant capacity.

Key words: green tea polyphenols, passive smoking, lung tissue, oxidative stress

中图分类号:

史春麟, 李晓焕, 黄翔翔. 绿茶多酚对被动吸烟引起小鼠肺氧化应激的干预研究[J]. 茶叶科学, 2018, 38(2): 212-220. doi: 10.13305/j.cnki.jts.2018.02.012.

SHI Chunlin, LI Xiaohuan, HUANG Xiangxiang. Effects of Green Tea Polyphenols on Oxidative Stress Induced by Passive Smoking in Mice Lung[J]. Journal of Tea Science, 2018, 38(2): 212-220. doi: 10.13305/j.cnki.jts.2018.02.012.

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绿茶多酚对被动吸烟引起小鼠肺氧化应激的干预研究

Effects of Green Tea Polyphenols on Oxidative Stress Induced by Passive Smoking in Mice Lung

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