为比较不同原料来源白茶的体外抗氧化和体内抗衰老作用并探究其作用机制,本研究制备白毫银针（Baihaoyinzhen, YZ）、白牡丹（Baimudan, MD）和寿眉（Shoumei, SM）的提取物,检测其主要功效成分含量及其体外抗氧化活性。通过诱导D-半乳糖衰老小鼠模型,探究其体内抗衰老作用。结果显示,YZ提取物的主要功效成分含量均高于MD和SM提取物,SM提取物的茶多酚和总黄酮含量高于MD提取物。在体外,相同浓度下YZ提取物抗氧化能力最强,MD和SM提取物差异不明显。在体内,YZ提取物在抑制肝脏脂质过氧化方面表现出显著优势（P<0.05）,SM提取物在提高血清抗氧化酶活性方面具有显著优势（P<0.05）,但未表现出剂量依赖关系。3种白茶提取物上调了小鼠肝脏抗氧化酶与抗衰老基因的表达,表现出剂量依赖性,有效地改善了机体氧化应激状态,且对小鼠均未表现出肝脏毒性作用。
To in vitro and in vivo evaluate and compare the antioxidant and anti-aging activities of white tea with different sources of raw materials, Baihaoyinzhen (YZ), Baimudan (MD), Shoumei (SM) were prepared and extracted to measure the main efficacy components. In vitro antioxidant assay, the antioxidantβactivitiesβwereβ also tested. For in vivo anti-aging test, the mice were subcutaneously injected with D-galactose and separately orally administered white tea extracts for aging model analysis. The contents of main efficacy components in YZ extract were higher than those in MD and SM extracts. The contents of tea polyphenols and flavonoids in SM extract were higher than those in MD extract. In vitro, YZ extract had better antioxidant capacity, and there was no obvious difference between SM and MD extract. In vivo, the degree of liver lipid peroxidation in YZH and YZL mice were significantly (P<0.05) decreased. The antioxidant enzyme activities were significantly (P<0.05) increased in serum of SMH and SML mice. But it did not show a dose-dependent manner. The 3 kinds of white tea extracts could up-regulate the expression levels of antioxidant enzymes and anti-aging genes to improve the oxidative stress state and it showed a dose-dependent manner. And there was no hepatotoxicity in these group mice.
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