研究了茶多酚（Tea Polyphenols,TP）降低肝脏中甘油三酯（Triglyceride, TG）含量从而对2型糖尿病性脂肪肝模型大鼠胰岛素抵抗的改善作用。健康SD大鼠分为6组：健康组、模型组、TP组、二甲双胍组、FL组和FL+TP组。所有大鼠在实验结束时测量体重、血清甘油三酯、肝脏中甘油三酯、空腹血糖、血清胰岛素等指标。TP组和二甲双胍组较模型组空腹血糖、血清胰岛素含量和胰岛素敏感指数下降;FL+TP组、TP组较FL组、模型组肝脏中甘油三酯含量下降。茶多酚可显著降低肝脏中的甘油三酯含量,调节脂代谢紊乱并改善胰岛素抵抗。
To study the effects of tea polyphenols (TP) on insulin resistance in the model rats of T2DM with NAFLD and its mechanism. Adult healthy SD rats were randomly divided into six equal groups: normal control (NC) group, model group, TP group, metformin group, fat liver (FL) group and FL+TP group. In the end of two weeks treatment, the levels of fasting serum glucose, insulin, TG and the TG of liver were measured. Two weeks after treatment, the fasting blood glucose and insulin and insulin sensitivity index of the TP group and metformin group were both significantly lower than those of the model group (both P<0.05). The TP group and FL+TP group compared with model group and FL group, the TP significantly reduce the levels of TG of the liver (both P<0.05). TP can efficiently ameliorated lipid metabolism in the liver tissue of rats and improve insulin sensitivity in the rat model of type 2 diabetes mellitus with fatty liver.
[1] Wolf G.Role of fatty acids in the development of insulin resistance and type 2 diabetes mellitus[J]. Nutr Rev, 2008, 66(10): 597-600.
[2] 中华医学会肝病学分会脂肪肝和酒精性肝病学组.非酒精性脂肪性肝病诊疗指南: 2010年修订版[J]. 中华肝脏病杂志, 2010, 18(3): 163-166.
[3] Wu LY,Juan CC, Hwang LS, et al. Green tea supplementation ameliorates insulin resistance and increases glucose transporter IV content in a fructose-fed rat mode[J]. Eur J Nutr, 2004, 43(2): 116-124.
[4] 俞河松, 竹建平. 茶多酚对2型糖尿病大鼠胰岛素抵抗的影响[J]. 海峡药学, 2009, 21(5): 31-32.
[5] 李伟光, 潘孝仁, Liuioja S, 等. 检测人群胰岛素敏感性的一项新指数[J]. 中华内科杂志, 1993, 32(10): 656.
[6] Isabelle Hininger-Favier, Rachida Benaraba, Sara Coves, et al. Green Tea Extract Decreases Oxidative Stress and Improves Insulin Sensitivity in an Animal Model of Insulin Resistance, the Fructose-Fed Rat[J]. Am CollNutr August, 2009, 28: 355-361.
[7] Nagle CA, Klett EL, Coleman RA.Hepatic triacylglycerol accumulation and insulin resistance[J]. J Lipid Res, 2009, 50: S74-S79.
[8] Dowman JK, Tomlinson JW, Newsome PN.Pathogenesis of nonalcoholic fatty liver disease[J]. QJM, 20l0, 103: 71-83.
[9] Chou CJ, Haluzik M, Gregory C, et al. WY14,643, a peroxisome proliferator-activated receptor alpha (PPARalpha) agonist, improves hepatic and muscle steatosis and reverses insulin resistance in lipoatrophic A-ZIP/F-1 mice[J]. J BiolChem, 2002, 277(27): 24484-24489.
[10] Michelle C Venables, Carl J Hulston, Hannah R Cox, et al. Green tea extract ingestion, fat oxidation, and glucose tolerance inhealthy humans[J]. Am J ClinNutr, 2008, 87: 778-784.
[11] Kim S Stote, David J Baer.Tea Consumption May Improve Biomarkersof Insulin Sensitivity and Risk Factorsfor Diabetes[J]. J Nutr, 2008, 138: 1584S-1588S.
[12] Yuko Hara, Masayuki Fujino, Masao Takeuchi, et al. Green-tea polyphenol (-)-epigallocatechin-3-gallate provides resistance to apoptosis in isolated islets[J]. HepatobiliaryPancreatSurg, 2007, 14: 493-497.
[13] Changhong Li, Aron Allen, Jae Kwagh, et al. Green Tea Polyphenols Modulate Insulin Secretion by Inhibiting Glutamate Dehydrogenase[J]. J Biol Chem, 2006, 281: 10214-10221.
[14] Gary Williamson.Possible effects of dietary polyphenols on sugarabsorption and digestion[J]. MolNutr Food Res, 2013, 57: 48-57.
[15] Kiran S Panickar.Effects of dietary polyphenols on neuroregulatory factorsand pathways that mediate food intake and energyregulation in obesity[J]. MolNutr Food Res, 2013, 57: 34-47.
[16] Wu LY, Juan CC, Ho LT, et al. Effect of green teasupplementation on insulin sensitivity in Sprague-Dawley rats[J]. AgricFood Chem, 2004, 52: 643-648.
[17] 王艳, 黄林, 钟英丽, 等. 三种食物多酚对化学诱导DM大鼠糖脂代谢的影响[J]. 营养学报, 2012, 34(6): 572-575, 581.
[18] Z F Zhang, Q Li, J Liang, et al. Epigallocatechin-3-O-gallate (EGCG) protects the insulin sensitivity in rat L6muscle cells exposed to dexamethasone condition[J]. Phytomedicine, 2010, 17: 14-18.
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