通过筛选流动相、优化洗脱梯度,建立了茶树紫色芽叶花青素HPLC的分析方法,并从提取的花青素粗制品中分离出9个主要色谱峰;运用HPLC-DAD-MS/MS联用技术,获得了其中7个组分的紫外-可见光谱信息、质谱数据,结合文献资料,初步推测了茶树紫色芽叶花青素中5个主要组分,分别为：飞燕草-3-半乳糖苷、矢车菊-3-半乳糖苷、飞燕草-3-芸香糖苷、矢车菊-3-芸香糖苷和天竺葵-3-芸香糖苷,此外,采用外标法对各组分进行了定量分析,并获得了粗提物,纯度为32%。该方法简便快捷,重现性好,可用于茶树紫色芽叶中花青素组分的分析与鉴定。
A rapid method for analysis of anthocyanins in purple buds and leaves of tea plant was established by high-performance liquid chromatography (HPLC) based on the screening of mobile phase and the optimization of elution gradient. Using the established HPLC method, nine major chromatographic peaks were separated from crude extracts of anthocyanins from purple buds and leaves of tea plant. Further, the ultraviolet-visible spectrum and mass spectrum data of seven out the nine components were obtained by HPLC-DAD-MS/MS analysis. Combined with the literature data, five major components of anthocyanins were identified as delphindin-3-galactoside, cyanidin-3-gal-actoside, delphindin-3-rutinoside, cyanidin-3-rutinoside, and pelargonium-3-rutinoside. Additionally, each component was quantitatively analyzed using an external standard method, and crude extracts of anthocyanins were obtained at the purity of 32%. The proposed method is convenient and rapid with high accuracy and reproducibility, which can be applied for determining anthocyanins in purple buds and leaves of tea plant.
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