研究L-茶氨酸对慢性不可预见性温和应激模型大鼠的抗抑郁作用及其机制。建立慢性不可预见性温和应激（CUMS）抑郁大鼠模型,通过糖水偏好实验,旷场实验,明暗箱实验评估L-茶氨酸的抗抑郁作用效果;为了阐释其相关机理,本研究通过酶联免疫法测定了各组大鼠海马和皮层组织中5-羟色胺（5-HT）、去甲肾上腺素（NA）、促肾上腺皮质激素（ACTH）和皮质酮（CORT）的含量,同时也检测了大鼠血清和海马组织中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。结果表明,与空白对照组相比,模型组大鼠糖水偏好度、横穿格数和直立次数、进入明箱中的大鼠数量以及停留的时间极显著降低,显示大鼠抑郁模型构建成功;而与模型组相比,灌胃低高两个剂量L-茶氨酸的大鼠抑郁症状有不同程度改善,尤其高剂量都达到显著水平。同时,L-茶氨酸能显著提升大鼠海马和皮层中5-HT、NA的含量,降低ACTH,CORT的含量,上调大鼠血清和皮层中SOD和GSH-Px的活性,进一步表明了其抗抑郁的功效,其机制可能主要与促进单胺类递质分泌有关。
The ameliorative effect of L-theanine on chronic unpredictable mild stress-induced depression in rats and the possible mechanism were investigated. The model of chronic unpredictable mild stress (CUMS) rats was established, and the antidepressant effect of L-theanine was evaluated through sucrose preference test, open-field test and light/dark box test. Then, the contents of the 5-hydroxytryptamine (5-HT), noradrenaline (NA), adrencocorticotropic hormone (ACTH) and corticosterone (CORT) in the hippocampus and prefrontal cortex were determined by ELISA analysis for exploring the underlying mechanism. Also, the enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in the serum and hippocampus were measured. The results showed that, compared with control group, the percentage of sucrose preference, the numbers of crossing and rearing, and the number of entries into light box and total duration time in model group were very significantly lower, which demonstrated the model was successfully established. Compared to the model group, the behavior of CUMS-induced depression was significantly ameliorated by the administration of L-theanine, especially that with high dose. Meanwhile, L-theanine could significantly increase the contents of 5-HT and NA, decrease the contents of ACTH and CORT in the hippocampus and prefrontal cortex of rats, increase the activities of SOD and GSH-Px in serum and prefrontal cortex, which further demonstrated the antidepressant function of L-theanine. The underlying mechanism might be mainly related to the enhanced secretion of monoamine neurotransmitter.
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