研究了茶叶提取物对体外培养的B16小鼠黑色素瘤细胞的生物学效应,并对其机理进行初步探讨。分别用茶黄素单体（TF1）、EGCG、纯度为40%的茶黄素（TF40）处理细胞,然后观察细胞形态,并以溴化二苯四偶氮法（MTT法）测定受试物对黑色素细胞活力的影响,以左旋多巴为底物测定细胞内酪氨酸酶活性,采用比色法测定细胞中的黑色素含量。实验结果表明,各化合物对B16黑色素瘤细胞形态有不同程度的影响,对细胞活性、酪氨酸酶浓度和黑色素合成量有浓度依赖性抑制作用。这3种茶提取物能通过多种途径抑制黑色素合成,从而达到美白的效果,且茶叶提取物的效果优于Vc。
The Bio-efficacy and mechanism of tea extract on mouse B16 melanoma cell in vitro were studied in this paper. Cells were treated with different concentration of TF1, EGCG and TF40, then the cell morphology was observed. The tyrosinase activity was determined by MTT method. The synthesis of melanin was determined using DOPA as substrate, and proliferation rate of B16 cells was determined by colorimetry, then analyzing the effects of drugs to these indexes. The results showed that the cell morphology was changed by the treatment of drugs. The tyrosinase activity, the synthesis of melanin and proliferation rate of B16 cells were all negatively related to the drugs concentration. Tea extracts and Vc can cause the inhibition of the synthesis of melanin, and resulted to white skin. The whiting effect of tea extracts is better than that of Vc.
[1] 王自强. 皮肤黑素成因分析与美白祛斑功效评价体系的研究[J]. 化学工程与装备, 2008(2): 14-18.
[2] Yang CS.Cancer prevention by tea: animal studies, molecular mechanisms and human relevance[J]. Nature, 2009, 6: 429-439.
[3] Tu YY, Tang AB, Watanabe N.The Theaflavin Monomers Inhibit the Cancer Cells Growth in Vitro[J]. Acta Biochimica et Biophysica Sinica, 2004, 36(7): 508-512.
[4] Xu Y, Jin YX, Wu YY, et al. Isolation and purification of four individual theaflavins using semi-preparative high performance liquid chromatography[J]. Journal of Liquid Chromatography & Releted Technologies, 2010, 33(20): 1791-1801.
[5] Chang TS, Lin CH.Melanogenesis inhibitory activity of two generic drugs: Cinnarizine and trazodone in mouse B16 cells[J]. International Journal of Molecular Sciences, 2011, 12: 8787-8796.
[6] Mei X, Wu YY, Mao X, et al. Antagonism of phenanthrene cytotoxicity for human embryo lung fibroblast cell line HFL-I by green tea polyphenols[J]. Envitonmental Pollution, 2011, 159: 164-168.
[7] Maeda K, Fukuda M, Arbutin. Mechanism of its depigmenting action in melanocyte culture[J]. J Pharmacol Exp Ther, 1996, 276(2): 765.
[8] 康琰琰, 张美英, 刑少璟, 等. 几种天然活性物对黑色素细胞毒性及美白功效的比较[J]. 日用化学品科学, 2005, 35(6): 361-363.
[9] Yamaguchi Y, Brenner M, Hearing VJ.The regulation of skin pigmentation[J]. Boil Chem, 2007, 282(38): 27557-27561.
[10] 宋琦如, 金锡鹏. 黑素代谢的分子生物学机制与美白剂的研究评价[J]. 日用化学品科学, 2000, 23(5): 193.
[11] 贾爱群, 孙洋, 王建新. 美白剂的发展现状与其黑色素抑制机理的研究进展[J]. 日用化学工业, 2001, 31(2): 41-44.
[12] Lin J, Hsiumei C, Lin Y, ,et al. Natural products with skin—whitening effects [J]. Journal of food. Natural products with skin—whitening effects [J]. Journal of food and drug analysis, 2008, 16(2): l-10.
[13] Wang KH, Lin RD, Hsu FL, et al. Cosmetic application of selected traditional Chinese herbal medicines[J]. Journal of Ethnopharmacology, 2006, 106(3): 353-359.
[14] 屠幼英. 茶与健康[M]. 西安: 世界图书出版社, 2011: 190-192.
[15] 张玮, 高静, 张敏, 等. 7种美白化合物对皮肤黑素细胞的体外生物学效应[J]. 药学实践杂, 2011, 29(1): 24-26, 40.
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