探讨六堡茶对高血脂症模型大鼠肠道短链脂肪酸的影响。以低剂量50 mg·kg-1·d-1、中剂量100 mg·kg-1·d-1和高剂量200 mg·kg-1·d-1六堡茶水提物对高脂模型大鼠进行干预性治疗4周后,收集大鼠粪便;将六堡茶醇提物与高脂模型空白组大鼠粪便进行体外发酵培养,最后采用气相色谱法分别测定粪便和发酵液短链脂肪酸含量。体内试验结果表明,六堡茶水提物能显著降低高脂大鼠肠道的乙酸含量,显著增加高脂大鼠肠道的丙酸和丁酸的含量;与绿茶水提物相比,六堡茶对高脂大鼠短链脂肪酸的影响更强。体外试验结果表明,六堡茶醇提物对乙酸的抑制率为16.92%,对丙酸和丁酸的促进率分别为24.81%和23.03%;4种茶叶中,六堡茶对肠道短链脂肪酸的影响最大,普洱熟茶次之,六堡原料毛茶和绿茶的影响最小;六堡茶醇提物不同极性段组分均能抑制乙酸分泌、促进丙酸和丁酸的分泌,其中,六堡茶醇提物通过60%的乙醇洗脱组分对肠道短链脂肪酸的影响最大。
The objective of this work was to investigate the effect of Liupao tea on levels of short chain fatty acid (SCFs) in intestinal tract of rats with hyperlipidemia. Firstly, rats with hyperlipidemia were fed with different dosages of Liupao tea water extract (low dosage, 50 mg·kg-1·d-1, medium dosage, 100 mg·kg-1·d-1 and high dosage, 200 mg·kg-1·d-1) for 4 weeks, collected the feces samples. Secondly, the Liupao tea ethanol extracts were cultured with fecal of model blank group in vitro. Finally, SCFAs in feces or fermentation broth were determined by gaschromatography (GC). The results in vivo show that Liupao tea water extract significantly reduced the level of acetate and increased propionate and butyrate in the intestinal tract of rats with hyperlipidemia. Compared with green tea water extract, the effect of Liupao tea water extract on levels of SCFAs in the intestinal tract of rats with hyperlipidemia was stronger. The results in vitro show that the inhibition rate of Liupao tea ethanol extract to acetate, the promotion rates of Liupao tea ethanol extract to propionate and butyrate were 16.92%, 24.81% and 23.03% respectively. Among the 4 types of tea, Liupao tea had the greatest influence on levels of the short-chain fatty acids in the intestine, followed by puer tea, and raw liupao tea and green tea had the smallest impact. Different polar components of Liupao tea alcohol extract can inhibit the production of acetate, promote the productions of propionate and butyrate. However, the effect of 60% alcohol elution component on the production of SCFAs in the intestinal tract of rats was the greatest.
[1] 郭慧玲. 肠道菌群与疾病关系的研究进展[J]. 微生物学通报, 2015, 42(2): 400-410.
[2] 刘松珍, 张雁, 张名位, 等. 肠道短链脂肪酸产生机制及生理功能的研究进展[J]. 广东农业科学, 2013, 40(11): 99-103.
[3] Piekarska J, Miśta D, Houszka M, et al.Trichinella spiralis: The influence of short chain fatty acids on the proliferation of lymphocytes, the goblet cell count and apoptosis in the mouse intestine[J]. Experimental Parasitology, 2011, 128(4): 419-426.
[4] Den Besten G, Havinga R, Bleeker A, et al.The short-chain fatty acid uptake fluxes by mice on a guar gum supplemented diet associate with amelioration of major biomarkers of the metabolic syndrome[J]. PLoS One, 2014, 9: e107392.
[5] 徐运杰, 方热军, 戴求仲. 短链脂肪酸的营养生理作用[J]. 饲料研究, 2007(8): 26-28.
[6] 付文政, 张春泽, 贾岩峰, 等. 粪便中短链脂肪酸检测及临床意义[J]. 中国肛肠病杂志, 2016, 36(4): 7-9.
[7] Mao Yan, Wei Bao Yao, Teng Jian Wen, et al.Analyses of fungal community by Illumina MiSeq platforms and characterization of Eurotium species on Liupao tea, a distinctive post-fermented tea from China[J]. Food Research International, 2017, 99: 641-649.
[8] 彭静静. 六堡茶的降血脂功能性研究[D]. 南宁: 广西大学, 2012.
[9] Yan Mao, Baoyao Wei, Jianwen Teng, et al.Polysaccharides from Chinese Liupao dark tea and their protective effect against Hyperlipidemia[J]. International Journal of Food Science and Technology, 2018, 53: 599-607.
[10] 黄丽, 彭静静, 夏宁, 等. 六堡茶调节高血脂症与抗凝血的功能特性研究[J]. 食品科技, 2013, 38(8): 123-128.
[11] Zhengmei Wu, Jianwen Teng, Li Huang, et al.Stability, antioxidant activity and in vitro bile acid-binding of green, black and dark tea polyphenols during simulated in vitro gastrointestinal digestion[J]. RSC Advances, 2015(5): 92089-92095.
[12] 赵园园, 黄丽, 韦保耀, 等. 六堡茶提取物对高脂小鼠肠道菌群的影响研究[J]. 食品工业科技, 2015, 36(21): 364-367.
[13] Condezo-Hoyos Luis, Mohanty Indira P, Noratto Giuliana D.Assessing non-digestible compounds in apple cultivars and their potential as modulators of obese faecal microbiota in vitro[J]. Food Chemistry, 2014, 161: 208-215.
[14] 明建, 吴素蕊, 曾凯芳, 等. 羊肚菌多糖PMEP-1对大鼠肠道内短链脂肪酸的影响[J]. 食品科学, 2010, 31(19): 367-371.
[15] Zhang Chenhong, Zhang Menghui, Pang Xiaoyan, et al.Structural resilience of the gut microbiota in adult mice under high-fat dietary perturbations[J]. The ISME Journal, 2012, 6(1): 1848-1857.
[16] Clarke Siobhan F, Murphy Eileen F, Sullivan Orla O, et al.Targeting the microbiota to address diet-induced obesity: a time dependent challenge[J]. Plos One, 2013, 8(6): e65790.
[17] 朱玲军, 项美香. 双歧杆菌三联活菌胶囊联合阿托伐他汀片对高脂血症患者血脂、载脂蛋白及肠道菌群水平的影响[J]. 中国微生态学杂志, 2016, 28(10): 1175-1177.
[18] R Ayesh, J A Weststrate, PN Drewitt, et al. Safety evaluation of phytosterol esters. Part 5. Faecal short-chain fatty acid and micro-ora content, faecal bacterial enzyme activity and serum female sex hormones in healthy normolipidaemic volunteers consuming a controlled diet either with or without a phytosterol ester-enriched margarine[J]. Food and Chemical Toxicology, 1999(37): 1127-1138.
[19] 吴水芸. 高脂肥胖对肠道微生态、短链脂肪酸的影响[D]. 镇江: 江苏大学, 2016.
[20] 李海珊, 刘丽乔, 聂少平. 茶多糖对小鼠肠道健康及免疫调节功能的影响[J]. 食品科学, 2017, 38(7): 187-192.
[21] 刘小玲, 李颖, 姜元欣, 等. 广西六堡茶主要特征成分分析[J]. 北京工商大学学报(自然科学版), 2012, 30(1): 46-50.
[22] 吴香兰. 黑茶改善小鼠胃肠道功能的实验研究[D]. 长沙: 湖南农业大学, 2013.
[23] Tomonori U, Naomi O.Green tea extract and black tea extract differentially influence cecal levels of short-chain fatty acids in rats[J]. Food Science & Nutrition, 2018, 6(4): 728-735.
[24] 杨阳, 张鑫, 翁佩芳, 等. 茶叶儿茶素的益生元活性研究[J]. 现代食品科技, 2015, 31(4): 128-136.
[25] 肖俊松, 单静敏, 曹雁平, 等. 多酚通过肠道菌群调节能量代谢研究进展[J]. 食品科学, 2012, 33(3): 300-303.
[26] Perry Rachel J, Peng Liang, Barry Natasha A, et al.Acetate mediates a microbiome-brain-β-cell axis to promote metabolic syndrome[J]. Nature, 2016, 534(7606): 213-217.
[27] 任傲, 童勤, 张彬. 短链脂肪酸的生成及其吸收机制[J]. 广东饲料, 2015, 24(10): 28-29.
[28] 张宁, 曾艳华, 吴希阳, 等. 中性大蒜果聚糖体外发酵产短链脂肪酸[J]. 食品与发酵工业, 2013, 39(1): 51-54.
[29] 杨德生, 康玉华, 李福春, 等. 结肠癌患者粪便胆汁酸和短链脂肪酸的变化及其意义[J]. 中华临床营养杂志, 2013, 24(4): 204-208.
[30] 傅红, 师英强, 莫善兢. 短链脂肪酸对人结肠癌Caco-2细胞增殖分化的影响与临床意义[J]. 中华消化杂志, 2003(8): 21-23.
[31] 沈晶晶, 董雯. 高脂肪与纤维饮食对大肠癌的影响研究[J]. 中国美容医学, 2011, 20(增2): 175.
浙公网安备 33019902000101号 
