利用高效液相色谱法分析了不同季节、不同茶树品种、不同部位茶鲜叶中16种多环芳烃（PAHs）的分布特性。结果表明,3个茶树品种,以政和大白茶鲜叶原料中PAHs的含量和标准偏差值最小,平均含量为126.92 μg·kg-1,偏差值为17.59 μg·kg-1;不同部位茶鲜叶中PAHs含量及标准偏差值表现为芽<第二叶<第四叶<第六叶,芽头PAHs含量为119.13 μg·kg-1,标准偏差值为14.36 μg·kg-1;不同季节茶鲜叶中PAHs含量及标准偏差值呈现秋<春<夏的分布特性,秋季茶鲜叶中PAHs的含量为112.75 μg·kg-1,标准偏差值为11.97 μg·kg-1;同时茶鲜叶中16种PAHs主要以2、3环PAHs为主,占PAHs总量的80%左右,4~6环PAHs相对较少。
The distribution characteristics of 16 Polycyclic Aromatic Hydrocarbon(PAHs) in different fresh tea leaves of different tea cultivars in different seasons were analyzed by using HPLC. Results showed that Zhenghedabaicha has lowest concentration of 16 PAHs and lowest standard deviation between Zhenghedabai, Taoyuandaye and Xiangfeicui, the average content is 126.92 μg·kg-1, the standard deviation is 17.59 μg·kg-1, the concentration of 16 PAHs and standard deviation in different part of tea leaves is expressed as: the bud< the second leaf< the fourth leaf< the sixth leaf, the PAHs′ concentration in bud is 119.13 μg·kg-1, standard deviation is 14.36 μg·kg-1. The concentration of 16 PAHs in different seasons is expressed as autumn<spring<summer in one year. The concentration of PAHs in autumn is 112.75 μg·kg-1, standard deviation is 11.97 μg·kg-1, 16 PAHs in fresh tea leaves are mainly composed of 2, 3 rings, account for about 80%. The proportion of 4-6 rings PAHs is lower.
[1] Doong R, Chang S, Sun Y.Solid-phase microextraction for determining the distribution of sixteen US Environmental Protection Agency polycyclic aromatic hydrocarbons in water samples[J]. Journal of Chromatography A, 2000, 879(2): 177-188.
[2] 程家丽, 黄启飞, 魏世强. 我国环境介质中多环芳烃的分布及其生态风险[J]. 环境工程学报, 2007, 1(4): 138-144.
[3] Tao S, Cui Y H, Xu F L, et al. Polycyclic aromatic hydrocarbons (PAHs) in agricultural soil and vegetables from Tianjin[J]. Science of the Total Environment, 2004, 320(1): 11-24.
[4] 刘向, 张干, 刘国卿. 南岭北坡苔藓中多环芳烃的研究[J]. 中国环境科学, 2005, 25(1): 101-105.
[5] 薛海全. 典型农作物中多环芳烃和多氯联苯的分布累积规律[D]. 泰安: 山东农业大学, 2011: 30-45.
[6] 宋玉芳. 土壤、植物样品中多环芳烃(PAHs)分析方法研究[J]. 应用生态学报, 1995, 6(1): 92-96.
[7] Schlemitz S, Pfannhauser W.Supercritical fluid extraction of mononitrated polycyclic aromatic hydrocarbons from tea- correlation with the PAH concentration[J]. Zeitschrift für Lebens- mitteluntersuchung und-Forschung A, 1997, 205(4): 305-310.
[8] 林道辉. 茶叶中多环芳烃的浓度水平、源解析及风险[D]. 杭州:浙江大学, 2005: 1-40.
[9] 沈菲. 钢铁工业区农田植物PAHs的浓度水平和影响因素[D]. 杭州:浙江大学, 2006: 8-10.
[10] 刘泓, 叶媛蓓, 唐玲. 植物对PAHs胁迫响应及植物修复研究进展[J]. 华侨大学学报: 自然科学版, 2008, 29(1): 1-5.
[11] 胡琳玲, 刘遵莹, 肖文军. 高效液相色谱法同时检测黑茶中16种多环芳烃化合物[J]. 茶叶科学, 2014, 34(4): 276-282.
[12] Simonich S L, Hites R A.Vegetation-atmosphere partitioning of polycyclic aromatic hydrocarbons[J]. Environmental science & technology, 1994, 28(5): 939-943.
[13] 周家斌, 王铁冠, 黄云碧. 北京部分地区大气PM10中多环芳烃的季节性变化[J]. 中国环境科学, 2005, 25(1): 115-119.
浙公网安备 33019902000101号 
