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黑茶对胎牛血清诱导脂肪变性的HepG2细胞的影响

  • 李苏 ,
  • 朱楠楠 ,
  • 刘仲华 ,
  • 蒋华军
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  • 1. 国家植物功能成分利用工程技术研究中心,湖南 长沙 410128;
    2. 湖南农业大学园艺园林学院茶学教育部重点实验室,湖南 长沙 410128
李苏(1986— ),男,湖南长沙人,硕士研究生,主要从事茶与天然产物功能成分研究。

收稿日期: 2012-08-13

  修回日期: 2012-11-12

  网络出版日期: 2019-09-04

基金资助

湖南省重大科技专项茯茶产业化关键技术研究与示范(08FJ1005)、国家茶叶产业技术体系资助项目(2011-2015)

Effect of Dark Tea on Fetal Bovine Serum Fat Accumulation in HepG2 Cells

  • LI Su ,
  • ZHU Nan-nan ,
  • LIU Zhong-hua ,
  • JIANG Hua-jun
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  • 1. National Research Center of Engineering Technology for Utilization of Functional Ingredients From Botanicals, Changsha 410128, China;
    2. Key Lab of Tea Science, College of Horticulture and Landscape, Hunan Agriculture University, Changsha 410128, China

Received date: 2012-08-13

  Revised date: 2012-11-12

  Online published: 2019-09-04

摘要

利用含有50%的胎牛血清的培养液处理HepG2细胞48βh,建立非酒精性脂肪变性细胞模型,分别从噻唑兰染色吸光度法(MTT值)、甘油三酯(TG)、胆固醇(TC)3个方面评价茯砖提取物(FZ)、六堡茶提取物(LB)、青砖茶提取物(QZ)、千两茶提取物(QL)清除肝细胞脂肪堆积的作用。结果表明,与模型组相比,加药处理组细胞的脂质堆积有缓解趋势,茯砖茶、六堡茶、千两茶、青砖茶提取物都可显著降低细胞内TG累积(P<0.01);而茯砖、青砖茶提取物对TC的清除效果较好(P<0.01),千两茶与六堡茶效果不明显。以上结果表明,黑茶提取物通过降低细胞内的脂类堆积,可能起到缓解脂肪变性的作用。

本文引用格式

李苏 , 朱楠楠 , 刘仲华 , 蒋华军 . 黑茶对胎牛血清诱导脂肪变性的HepG2细胞的影响[J]. 茶叶科学, 2013 , 33(1) : 45 -52 . DOI: 10.13305/j.cnki.jts.2013.01.004

Abstract

Fu brick tea extract(FZ), Liubao tea extract(LB), Qing brick tea extract(QZ), Qianliang tea extract(QL) were assessed for the hepatic lipid-lowering potential when administered conditions contained 50% FBS in 48βh to establish the model of hepatic steatosis in vitro. MTT value, total triglyceride (TG), total cholesterol(TC) were used to evaluate the effect of dark tea on HepG2 steatosis. Compared with the model groups, cellular lipid accumulation was significantly suppressed in the treatment groups. Moreover,FZ, LB, QZ, QL all showed highly significant inhibitory effect against TG accumulation(P<0.01). FZ, QZ also significantly reduced the TC accumulation (P<0.01), however, the effect of LB and QL were obvious. These results showed dark tea extracts might reduce lipid accumulation in cell. It showed the suppressive effect on lipid accumulation.

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