利用数字基因表达谱技术研究了温湿度变化对秋插茶苗芽萌发基因表达的影响。通过保温棚处理日均温度提高约5.15℃,湿度提高5.10%,两者茶苗芽萌发具有明显差异。比较保温棚与对照芽的基因表达共筛选获得949个差异表达基因,其中503个基因受保温棚处理诱导上调表达,446个基因下调表达。对差异表达基因进行GO分类分析,在P值<0.01的情况下,有360个基因与14个GO分类匹配。其中7个GO分类与光合作用相关,P值最小的GO分类为光系统,其11个基因均表现为上调表达,说明高温高湿条件具有促进光合相关基因表达的作用。这些结果将为深入研究理解茶苗的生长机制及相关基因的克隆打下基础。
Differences of gene expression in buds of tea cuttings in response to temperature and humidity changes were studied. The daily temperature and humidity increased by 5.15℃ and 5.10% under plastic shading treatment. Meanwhile, bud expansion of tea cuttings under plastic shading was clearly different from that of control. After digital gene expression profiling analysis, a total of 949 differential expressed genes were obtained, including 503 up- and 446 down-regulated genes from buds of tea cuttings in plastic shed. Gene Ontology (GO) analysis showed that 360 genes were classified into 14 GO terms when P value <0.01. Seven GO terms were associated with photosynthesis. The GO term with the least P value was related to photosystem, which suggest high temperature and humidity are favorable for the induction of genes associated with photosynthesis. This study might provide new insight into understanding the mechanism of rapid propagation and related gene cloning in Camellia sinensis.
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