欢迎访问《茶叶科学》,今天是

生物酶在茶叶提取加工技术中的应用研究

  • 龚玉雷 ,
  • 魏春 ,
  • 王芝彪 ,
  • 沈雪亮
展开
  • 浙江工业大学 生物与环境工程学院,浙江 杭州 310014
龚玉雷(1988— ),男,浙江平湖人,硕士研究生,主要从事酶与茶叶方面的研究

收稿日期: 2012-11-26

  修回日期: 2013-03-04

  网络出版日期: 2019-09-04

Research Progress of Cellulase and Pectinase in the Extraction of Tea Leaves

  • GONG Yu-lei ,
  • WEI Chun ,
  • WANG Zhi-biao ,
  • SHEN Xue-liang
Expand
  • Zhejiang University of Technology, College of Biological & Environmental Engineering, Hangzhou 310014, China

Received date: 2012-11-26

  Revised date: 2013-03-04

  Online published: 2019-09-04

摘要

茶是一种广受欢迎的软饮料,其中滋味醇浓且茶乳酪少的茶饮是最受青睐的。现代生物技术将外源生物酶应用于茶叶提取中,有利于茶叶细胞壁的浸渍、蛋白质的水解和茶乳酪的转溶,增加茶叶中茶多酚、茶黄素和氨基酸等的得率,改善茶叶色、香、味等品质。本文详细介绍了纤维素酶、果胶酶、单宁酶和蛋白酶等生物水解酶,并对这些酶在茶叶提取中的应用进行综述,同时对复合酶系在茶叶提取加工中的发展趋势进行了展望。

本文引用格式

龚玉雷 , 魏春 , 王芝彪 , 沈雪亮 . 生物酶在茶叶提取加工技术中的应用研究[J]. 茶叶科学, 2013 , 33(4) : 311 -321 . DOI: 10.13305/j.cnki.jts.2013.04.004

Abstract

Tea is one of the most widely consumed soft beverages. Tea beverages more excellent in flavor and formation of less deposits are more preferred. With the development of modern biotechnology, the microbial enzymes are applied in the extraction process of tea leaves. The cell walls of tea leaf and proteins are easy to be hydrolyzed, and the formation of precipitates can be avoided. The extract yields of polyphenols, thearubigins and amino acids were increased. And the tea qualities including color, odour and flavor improved accordingly. In this article, the hydrolytic enzymes including cellulase, pectinase, tannase and protease are introduced in detail, and their applications in tea extraction process are reviewed. The prospects of complex enzymes system in tea extraction process are also previewed.

参考文献

[1] 杨贤强, 王岳飞, 陈留记, 等. 茶多酚化学[M]. 上海: 上海科技出版社, 2003: 109-116.
[2] Quansheng Chen, Zhiming Guo, Jiewen Zhao.Identification of green tea′s [Camellia sinensis (L.)] quality level according to measurement of main catechins and caffeine contents by HPLC and support vector classification pattern recognition[J]. Journal of Pharmaceutical and Biomedical Analysis, 2008, 48: 1321-1325.
[3] Alcázar A, Ballesteros O, Jurado J M.Differentiation of Green, White, Black, Oolong, and Pu-erh Teas According to Their Free Amino Acids Content[J]. J Agric Food Chem, 2007, 55: 5960-5965.
[4] Carpita N, Gibeaut D.Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth[J]. The Plant Journal, 1993, 3: 1-30.
[5] Harold N, Graham Ph D.Green tea composition, consumption, and polyphenol chemistry[J]. Preventive Medicine, 1992, 21: 334-350.
[6] Tomme P, Warren R A J, Gilkes N R. Cellulose hydrolysis by bacteria and fungi[J]. Advances In Microbial Physiology, 1995, 37: 1-81.
[7] Woodward J.Synergism in cellulase systems. Bioresource Technology[J]. 1991, 36: 67-75.
[8] 杨文雄, 高彦祥. 酶对改善茶汤萃取品质的研究[J]. 食品工业科技, 2005, 26(4): 61-63, 67.
[9] 梁靖, 须海荣, 蒋文莉, 等. 纤维素酶在速溶茶中的应用研究[J]. 茶叶, 2002, 28(1): 25-28.
[10] 李凤娟,丁兆堂, 杜金华. 低温浸提茶汁的最优工艺条件[J]. 食品与发酵工业, 2004, 30(4): 121-124.
[11] Jae Hun Kim, Jeong Hoon Pan, Wan Heo, et al. Effects of Cellulase from Aspergillus niger and Solvent Pretreatments on the Extractability of Organic Green Tea Waste[J]. Journal of Agricultural and Food Chemistry, 2010, 58: 10747-10751.
[12] 汪珈慧, 李燕, 姚紫涵, 等. 利用固定化纤维素酶酶解夏季绿茶工艺的研究[J]. 茶叶科学, 2012, 32(1): 37-43.
[13] 姜绍通, 潘丽军, 郑志, 等. 酶法提取速溶绿茶的研究[J]. 农业工程学报, 1998, 14(4): 234-238.
[14] Yoshihiro K, Shizuka S, Rie K, ,et al. Tea essence. Tea essence and method for producing tea aroma: Japan, 075112[P].2006-03-23.
[15] Su Erzheng, Xia Tao, Gao Liping, et al. Immobilization of β-glucosidase and its aroma-increasing effect on tea beverage[J]. Food and Bioproducts Processing, 2010, 88: 83-89.
[16] Sakai T, Sakamoto T, Hallaert J, et al. Pectin, pectinase and protopectinase: production, properties, and applications[J]. Advances in Applied Microbiology, 1993, 39: 213-294.
[17] Jayani R S, Saxena S, Gupta R.Microbial pectinolytic enzymes: A review[J]. Process Biochemistry, 2005, 40(4): 2931-2944.
[18] Ronald P de Vries, Jaap Visser. Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides[J]. Microbiology and Molecular Biology Reviews, 2001, 65(4): 497-522.
[19] Chandini S K, Rao L J, Gowthaman M K, et al. Subramanian R. Enzymatic treatment to improve the quality of black tea extracts[J]. Food Chemistry, 2011, 127: 1039-1045.
[20] 尹军峰, 钱晓军, 罗龙新, 等. 果胶酶提高茶汁膜分离性能的研究[J]. 饮料工业, 2003(6): 30-33.
[21] 黄静, 肖文军. 外源果胶酶对绿茶液微滤过程的效应研究[J]. 茶叶通讯, 2006, 33(3): 10-14.
[22] Sanderson G W, Englewood N J, Simpson SW. Pectinase enzyme treating process for preparing high bluk density tea powders: United States, 3787582[P].1974-01-22.
[23] Saito K, Hiramoto T. Tea extract and method for producing the same: United States, 0274788[P].2011-11-10.
[24] Omae Hideo. Green Tea Beverage and Method for Producing the Same: Japan, 119209[P].2002-04-23.
[25] Kinugasa K, Takeo K.Deterioration mechanism for tea infusion aroma by retort pasteurization[J] Agricultural Biology and Chemistry, 1990, 54(10): 2537-2542.
[26] 刘菁, 陶文沂. 酶法制取茶树废弃老叶中的茶风味物质[J]. 食品与发酵工业, 2007, 33(3): 158-160.
[27] 周小玲, 汪东风, 李素臻, 等. 不同酶法提取工艺对茶多糖组成的影响[J]. 茶叶科学, 2007, 27(1): 27-32.
[28] 傅博强, 谢明勇, 周鹏, 等. 纤维素酶法提取茶多糖[J]. 无锡轻工大学学报, 2002, 21(4): 362-366.
[29] Rodríguez-Durán LV, Valdivia-Urdiales B, Contreras-Esquivel JC, et al. Contreras-Esquivel. Novel Strategies for Upstream and Downstream Processing of Tannin Acyl Hydrolase[J]. Enzyme Research, 2011: 1-20.
[30] 许勇泉, 尹军峰. 绿茶茶汤冷后浑特性研究[J]. 茶叶科学, 2010, 30(增刊1): 527-532.
[31] Tsai C H, Ohio C. Enzymatic treatment of black tea leaf: United States, 4639375[P].1987-01-27.
[32] Min JerLu, Sheng CheChu, Lipyng Ya, et al. Effect of tannase treatment on protein-tannin aggregation and sensory attributes of green tea infusion[J]. LWT-Food Science and Technology, 2009, 42: 338-342.
[33] Maruyama Eizo, Sugiyama, Yukiteru, ,et al. Process for producing purified tea extract: USA. Process for producing purified tea extract: USA, 12/281402[P].2009-12-02.
[34] Min JerLu, Chinshuh Chen. Enzymatic modification by tannase increases the antioxidant activity of green tea[J]. Food Research International, 2008, 42: 130-137.
[35] Chen Z Y, Chan P T.Antioxidative activity of green tea catechins in canola oil[J]. Chemistry and Physics of Lipids, 1996, 82(2): 163-172.
[36] Min JerLu, Chinshuh Chen. Enzymatic tannase treatment of green tea increases in vitroinhibitory activity against N-nitrosation of dimethylamine[J]. Process Biochemistry, 2007, 42: 1285-1290.
[37] 朱旗, 施兆鹏. 环糊精在茶叶加工申的应用[J]. 中国茶叶加工, 1998(4): 14-16.
[38] 孙庆磊, 孔俊豪, 陈小强, 等. 聚乙烯聚吡咯烷酮(PVPP)在茶饮料沉淀控制中应用研究进展[J]. 中国茶叶加工, 2011, 2: 29-32.
[39] Boadi D K, Neufeld R J.Encapsulation of tannase for the hydrolysis of tea tannins[J]. Enzyme and Microbial Technology, 2001, 28:590-595.
[40] Su Erzheng, Xia Tao, GaoLiping, et al. Immobilization and Characterization of Tannase and its Haze-removing[J]. Food Science and Technology International, 2009, 15: 545-552.
[41] 顾谦, 陆锦时, 叶宝存. 茶叶化学[M]. 北京: 中国科学技术大学出版社, 2002.
[42] 郑宝东, 曾绍校. 酶处理对绿茶浸提液成分及膜过滤通量影响的研究[J]. 农业工程学报, 2003(6): 212-214.
[43] KAWABATA CHOKO, SAKAMAKI SHIZUKA, KAWAGUCHI RIE, et al. Changes in amino acids and volatile compounds in tea extracts by treating with enzymes[J]. Koryo, Terupen oyobi Seiyu Kagaku ni kansuru Toronkai Koen Yoshishu, 2003, 47: 88-90.
[44] 孙素. 酶处理对绿茶提取液品质影响的研究[D]. 杭州: 浙江大学, 2006.
[45] 倪君. 绿茶中非水溶性蛋白及酶解效果的研究[D]. 杭州:浙江大学, 2007.
[46] Lekh RJ, Djong-Chi C, Tsutomu O, et al. L-theanine-a unique amino acid of green tea and its relaxation effection humans[J]. Trends Food Sci Tech, 1999, 10: 199-204.
[47] Xu Yongquan, Zhong Xiaoyu, Chen Suqin, et al. Hydrolysis of green tea residue protein using proteolytic enzyme derived from Aspergillus oryzae[J]. J Food Sci Technol, 2011.
[48] Asano K, Shinagawa K, Hashimoto N, et al. Charaeterization of haze-forming proteins of beer and their roles in chill haze-formation[J]. J Am Soc Brew Chem, 1982, 40: 147-154.
[49] Asquith T, Butler L.Interaction of condensed tannins with selected Proteins[J]. Phytochemistry, 1986, 25(7): 1591-1593.
[50] 黄惠华, 王少斌, 王志, 等. 茶多酚-蛋白质之间的络合及沉淀回收研究[J]. 食品科学, 2002, 23: 26-30.
[51] 谭淑宜. 提高速溶茶品质的研究I酶法提取[J]. 湖南农学院学报, 1991, 17(4): 708-713.
[52] 武永福, 张宁. 酶在速溶绿茶浸提中的应用研究[J]. 粮油加工, 2010, 10: 105-108.
[53] Atsushi M, Shunsuke U, Yoshiharu S. Method for producing tea extract. Japan 125477[P].2008-06-05.
[54] Shinji K, Masao K, Shiyoujirou H. Method for producing extracted solution from tea leaf and method for producing tea drink using the extracted solution from the tea leaf: Japan, 210110[P].2003-07-29.
[55] Ben-Shalon, N. Hindrance of hemicellulose and cellulose hydrolysis by pectic substances[J]. Journal of Food Science, 1986, 51: 720-721, 730.
[56] 张卫红, 张效林. 复合酶法提取茶多酚工艺条件研究[J]. 食品研究与开发, 2006, 27(11): 5-7.
[57] 舒红英, 罗旭彪, 王永珍. 绿茶中茶多酚的复合酶-微波法提取工艺研究[J]. 中草药, 2011, 42(7): 1309-1302.
[58] Lehmberg G L, Balentine D A, Hang R S, ,et al. Extraction process of tea with enzymes: United States. Extraction process of tea with enzymes: United States, 5925389[P].1999-07-20.
[59] Lehmberg G L, Balentine D A, Hang R S, ,et al. Enzyme extraction process for tea: United States. Enzyme extraction process for tea: United States, 5952023[P].1999-09-04.
[60] Lehmberg G L, Balentine D A, Hang R S, ,et al. Enzyme extraction process for tea: United States. Enzyme extraction process for tea: United States, 5919500[P].1999-07-06.
[61] Shinichiro T, Tetsuya F. Method for producing tea leaf extract, tea leaf extract and tea drink: Japan, 050271[P].2011-03-17.
[62] 丁兆堂, 王秀峰, 于海宁, 等. 茶多酚固定化酶体外氧化产物茶黄素组成及其化学发光分析[J]. 茶叶科学, 2005, 25(1): 49-55.
文章导航

/