利用cDNA-AFLP技术研究了特异茶树品种“紫娟”幼嫩叶片和成熟叶片的基因表达差异。从256对引物组合中获得59个差异表达带,其中在幼嫩叶片中获得26个上调片段,成熟叶片中获得33个上调片段。通过GenBank BLASTX比对分析,所获得的片段包括转录因子、代谢相关蛋白、信号蛋白以及一些假设蛋白、未知蛋白和没有比对的基因片段。利用RT-PCR对片段ZM4、ZM7、ZM12进行表达特性分析表明,ZM4、ZM7、ZM12均在成熟叶片中上调表达。这些研究结果将为深入研究理解“紫娟”茶树叶色转变的机理,以及相关基因克隆打下基础。
Differences of gene expression between young and mature leaves of tea plant (Camellia sinensis var. assamica, cultivar Zijuan) were studied by cDNA-AFLP. The results showed that 59 Transcript-Derived Fragments (TDFs) were obtained, including 26 up-regulated TDFs from young leaves, and 33 up-regulated TDFs from mature leaves. According to blasting results, these TDFs were included signal transduction genes, transcription factors, primary metabolism genes, putative protein and unknown protein and no significant homology found. RT-PCR was used to analyze the expression of ZM4, ZM7, ZM12, showed that expression of these ZM4, ZM7, ZM12 increased obviously in mature leaves. This study established a basis for further understanding the purple mechanism of tea leaves in cultivar “Zijuan” and related gene cloning.
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