HPLC-APCI-MS法测定茶叶中的茶氨酸

王虹; 康海宁; 陈军辉; 赵恒强; 王小如; 黎先春

doi:10.13305/j.cnki.jts.2009.1.004

茶叶科学 >

2009 , Vol. 29 >Issue 1: 20 - 26

DOI: https://doi.org/10.13305/j.cnki.jts.2009.1.004

HPLC-APCI-MS法测定茶叶中的茶氨酸

王虹 ,
康海宁 ,
陈军辉 ,
赵恒强 ,
王小如 ,
黎先春

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1. 国家海洋局第一海洋研究所青岛市现代分析技术及中药标准化重点实验室,山东青岛 266061;
2. 厦门大学化学化工学院化学系现代分析科学教育部重点实验室,福建厦门 361005;
3. 上海水产大学生命学院,上海 200090

王虹（1978— ）,女,新疆阿克苏人,硕士,主要从事天然产物分析化学研究。

收稿日期: 2008-06-26

修回日期: 2008-09-08

网络出版日期: 2019-09-06

基金资助

福建省科技厅茶叶指纹图谱质量控制研究项目（2006N0043）和海洋一所基本科研业务专项（GY-022008T32）资助

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Determination of Theanine in Tea by High Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization Mass Spectrometry

WANG Hong ,
KANG Hai-ning ,
CHEN Jun-hui ,
ZHAO Heng-qiang ,
WANG Xiao-ru ,
Frank Sen-Chun Lee

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1. QingDao Key Lab on Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography of SOA, Qingdao 266061, China;
2. Department of Chemistry and the Key Laboratory of Analytical Science of the MOE, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
3. College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China

Received date: 2008-06-26

Revised date: 2008-09-08

Online published: 2019-09-06

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摘要

采用高效液相色谱-大气压化学电离质谱（HPLC-APCI-MS）联用技术,建立了茶叶中茶氨酸的含量测定方法。实验采用C₈柱,以甲醇-水为流动相,等度洗脱,采用正离子大气压化学电离质谱（SIM模式）进行测定,可以获得稳定的质谱信号,并采用正交实验设计对APCI-MS仪器条件进行了优化。结果表明,本文建立的HPLC-APCI-MS方法操作简便、重现性好,灵敏度较紫外检测器有很大的提高,可用于微量茶氨酸的快速测定。

关键词： 茶叶; 茶氨酸; 高效液相色谱-大气压化学电离质谱; 选择离子监测模式; 正交实验

本文引用格式

王虹 , 康海宁 , 陈军辉 , 赵恒强 , 王小如 , 黎先春 . HPLC-APCI-MS法测定茶叶中的茶氨酸[J]. 茶叶科学, 2009 , 29(1) : 20 -26 . DOI: 10.13305/j.cnki.jts.2009.1.004

Abstract

HPLC coupled online with atmospheric pressure chemical ionization MS technique was developed for the qualitative and quantitative determination of theanine in tea. The theanine and other compounds in the tea extract were separated on an Alltima C₈ column with methanol and water (10:90 v/v) as mobile phase, with flow rate of 0.6 ml/min and APCI-MS SIM m/z 175.1 detection. In the positive APCI mode, sensitive and stable MS signal could be obtained by APCI-MS (SIM m/z 175.1) detection, and the APCI-MS conditions were also optimized by orthogonal design. The results indicated that HPLC-APCI-MS method was simple, sensitive and reliable for the determination of theanine in tea. It was important that the detection sensitivity was improved greatly by APCI-MS than UV. Eight tea samples from Fujian province were analysed using the established methods.

Key words： tea; theanine; HPLC-APCI-MS; SIM mode; orthogonal experiment

参考文献

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