[1] Facchin PJ.Alkaloid biosynthesis in plants: biochemistry, cell biology, molecular regulation, and metabolic engineering applications[J]. Annu Rev Plant Physiol Plant Mol Biol, 2001, 52: 29-66.
[2] Anaya AL, Cruz-Ortega R, Waller GR.Metabolism and ecology of purine alkaloids[J]. Front Biosci, 2006, 11: 2354-2370.
[3] Ashihara H.Metabolism of alkaloids in coffee plants[J]. Braz J Plant Physiol, 2006, 18(1): 1-8.
[4] Ashihara H, Crozier A Biosynthesis and metabolism of caffeine and related purine alkaloids in plants[J]. Adv Bot Res, 1999, 30: 118-205.
[5] Senanayake UM, Wijesekera OB.Theobromine and caffeine content of the cocoa bean during its growth[J]. J Sci Food Agric, 1971, 22(5): 262-263.
[6] Zheng XQ, Koyama Y, Nagai C, et al. Biosynthesis, accumulation and degradation of theobromine in developing Theobroma cacao fruits[J]. J Plant Physiol, 2004, 161(4): 363-369.
[7] Ashihara H, Kato M, Ye C-X.Biosynthesis and metabolism of purine alkaloids in leaves of Cocoa tea (Camellia ptilophylla)[J]. J Plant Re, 1998, 111(4): 599-604.
[8] 叶创兴, 林永成, 苏建业, 等. 苦茶的嘌呤生物碱[J]. 中山大学学报:自然科学版, 1999, 38(5): 82-86.
[9] Nagata T, Sakai S.Differences in caffeine, flavanols and amino acids contents in leaves of cultivated species of Camellia[J]. Jpn J Breed, 1986, 34(4): 459-467.
[10] Fujimori N, Ashihara H.Adenine metabolism and the synthesis of purine alkaloids in flowers of Camellia[J]. Phytochemistry, 1990, 29(11): 3513-3516.
[11] Ashihara H, Kubota H.Patterns of adenine metabolism and caffeine biosynthesis in different parts of tea seedlings[J]. Physiol Plantarum, 1986, 68(2): 275-281.
[12] Mazzafera P, Carvalho A.Breeding for low seed caffeine content of coffee (Coffea L.) by interspecific hybridization[J]. Euphytica, 1992, 59(1): 55-60.
[13] Zheng XQ, Ashihara H.Distribution, biosynthesis and function of purine and pyridine alkaloids in Coffea arabica seedlings[J]. Plant Sci, 2004, 166(3): 807-813.
[14] Mazzafera P.Caffeine, theobromine and theophylline distribution in Ilex paraguariensis[J]. Braz J Plant Physiol, 1994, 6: 149-151.
[15] Athayde ML, Coelho GC, Schenkel EP.Caffeine and theobromine in epicuticular wax of Ilex paraguariensis A. St.-Hil[J]. Phytochemistry, 2000, 55(7): 853-857.
[16] Baumann TW, Schulthess BH, Hänni K.Guaraná (Paullinia cupana) rewards seed dispersers without intoxicating them by caffeine[J]. Phytochemistry, 1995, 39(5): 1063-1070.
[17] Meurer-Grimes B, Berkov A, Beck H.Theobromine, theophylline, and caffeine in 42 samples and products of guaraná (Paullinia Cupana, Sapindaceae)[J]. Econ Bot, 1998, 52(3): 293-301.
[18] Weckerle CS, Stutz MA, Baumann TW.Purine alkaloids in Paullinia[J]. Phytochemistry, 2003, 64(3): 735-742.
[19] Kretschmar JA, Baumann TW.Caffeine in Citrus flowers[J]. Phytochemistry, 1999, 52(1): 19-23.
[20] Ashihara H, Crozier A.Caffeine: a well known but little mentioned compound in plant science[J]. Trends Plant Sci, 2001, 6(9): 407-413.
[21] 张宏达, 叶创兴, 张润梅, 等. 中国发现新的茶叶资源——可可茶[J]. 中山大学学报: 自然科学版, 1988(3): 131-133.
[22] Nagata T, Sakai S.Purine base pattern of Camellia irrawadiensis[J]. Phytochemistry, 1985, 24(10): 2271-2272.
[23] Fujimori N, Ashihara H.Biosynthesis of theobromine and caffeine in developing leaves of Coffea arabica[J]. Phytochemistry, 1994, 36(6): 1359-1361.
[24] Rakotomalala JJ, Cros E, Clifford MN, et al. Caffeine and theobromine in green beans from Mascarocoffea[J]. Phytochemistry, 1992, 31(4): 1271-1272.
[25] Koyama Y, Tomoda Y, Kato M, et al. Metabolism of purine bases, nucleosides and alkaloids in theobromine-forming Theobroma cacao leaves[J]. Plant Physiol Biochem, 2003, 41(11/12): 977-984.
[26] Petermann JB, Baumann TW.Metabolic relations between methylxanthines and methyluric acids in Coffea L.[J]. Plant Physiol, 1983, 73(4): 961-964.
[27] Hammerstone Jr JF, Romanczyk Jr LJ, Aitkent WM.Purine alkaloid distribution with Herrania and Theorbroma[J]. Phytochemistry, 1994, 35(5): 1237-1240.
[28] Baumann TW, Wanner H.The 1,3,7,9-tetramethyluric acid content of cupu (Theobroma grandiflorum)[J]. Acta Amazon, 1980, 10: 425.
[29] Ashihara H.Purine metabolism and the biosynthesis of caffeine in maté leaves[J]. Phytochemistry, 1993, 33(6): 1427-1430.
[30] Ashihara H, Suzuki T.Distribution and biosynthesis of caffeine in plants[J]. Front Biosci, 2004, 9: 1864-1876.
[31] Suzuki T, Ashihara H, Waller GR.Purine and purine alkaloid metabolism in Camellia and Coffea plants[J]. Phytochemistry, 1992, 31(8): 2575-2584.
[32] Ashihara H, Monteiro AM, Gillies FM, et al. Biosynthesis of caffeine in leaves of coffee[J]. Plant Physiol, 1996, 111(3): 747-753.
[33] Kato M, Kanehara T, Shimizu H, et al. Caffeine biosynthesis in young leaves of Camellia sinensis: in vitro studies on N-methyltransferase activity involved in the conversion of xanthosine to caffeine[J]. Physiol Plantarum, 1996, 98(3): 629-636.
[34] Uefuji H, Ogita S, Yamaguchi Y, et al. Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants[J]. Plant Physiol, 2003, 132(1): 372-380.
[35] 宛晓春. 茶叶生物化学: 第三版[M]. 北京: 中国农业出版社, 2003: 80.
[36] Koshiishi C, Kato A, Yama S, et al. A new caffeine biosynthetic pathway in tea leaves: utilisation of adenosine released from the S-adenosyl-methionine cycle[J]. FEBS Lett, 2001, 499(1/2): 50-54.
[37] Ashihara H, Gillies FM, Crozier A.Metabolism of caffeine and related purine alkaloids in leaves of tea (Camellia sinensis L.)[J]. Plant Cell Physiol, 1997, 38(4): 413-419.
[38] Zheng XQ, Ye CX, Kato M, et al. Theacrine (1,3,7,9-tetramethyluric acid) synthesis in leaves of a Chinese tea, kucha (Camellia assamica var. kucha)[J]. Phytochemistry, 2002, 60(2): 129-134.
[39] Negishi O, Ozawa T, Imagawa H.Methylation of xanthosine by tea-leaf extracts and caffeine biosynthesis[J]. Agric Biol Chem, 1985, 49(3): 887-890.
[40] Moisyadi S, Neupane KR, Stiles JI.Cloning and characterization of a cDNA encoding xanthosine-N7-methyltransferase from coffee (Coffea arabica[J]. Acta Hortic, 1998, 461: 367-378.
[41] Mizuno K, Kato M, Irino F, et al. The first committed step reaction of caffeine biosynthesis: 7-methylxanthosine synthase is closely homologous to caffeine synthases in coffee (Coffea arabica L.)[J]. FEBS Lett, 2003, 547(1/3): 56-60.
[42] Negish O, Ozawa T, Imagawa H.N-methylnucleosidase from tea leaves[J]. Agric Biol Chem, 1988, 52(1): 169-175.
[43] Suzuki T, Takahashi E.Biosynthesis of caffeine by tea-leaf extracts. Enzymic formation of theobromine from 7-methylxanthine and of caffeine from theobromine[J]. Biochem J, 1975, 146(1): 87-96.
[44] Kato M, Mizuno K, Fujimura T, et al. Purification and characterization of caffeine synthase from tea leaves[J]. Plant Physiol, 1999, 120(2): 579-586.
[45] Kato M, Mizuno K, Crozie A, et al. Caffeine synthase gene from tea leaves[J]. Nature, 2000, 406(6799): 956-957.
[46] Mizuno K, Tanaka H, Kato M, et al. cDNA cloning of caffeine (theobromine) synthase from coffee (Coffea arabica L.)[C]//Association Scientifique Internationale du Café. International Scientific Colloquium on Coffee. Paris:Association Scientifique Internationale du Café, 2001: 815-818.
[47] Ogawa M, Herai Y, Koizumi N, et al. 7-Methylxanthine methyltransferase of coffee plants[J]. J Biol Chem, 2001, 276(11): 8213-8218.
[48] McCarthy AA, Biget L, Lin C, et al. Cloning, expression, crystallization and preliminary X-ray analysis of the XMT and DXMT N-methyltransferases from Coffea canephora (robusta)[J]. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007, 63: 304-307.
[49] Mizuno K, Okuda A, Kato M, et al. Isolation of a new dual-functional caffeine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to caffeine from coffee (Coffea arabica L.)[J]. FEBS Lett, 2003, 534(1/3): 75-81.
[50] 冯艳飞, 梁月荣. 茶树S-腺苷甲硫氨酸合成酶基因的克隆和序列分析[J]. 茶叶科学, 2001, 21(1): 21-25.
[51] Keya CA, Crozier A, Ashihara H.Inhibition of caffeine biosynthesis in tea (Camellia sinensis) and coffee (Coffea arabica) plants by ribavirin[J]. FEBS Lett, 2003, 554(3): 473-477.
[52] 李叶云, 芦原坦. 茶树咖啡碱生物合成相关酶基因表达研究[J]. 中国茶叶, 2009, 31(6): 20-21.
[53] Yoneyama N, Morimoto H, Ye CX, et al. Substrate specificity of N-methyltransferase involved in purine alkaloids synthesis is dependent upon one amino acid residue of the enzyme[J]. Mol Genet Genomics, 2006, 275(2): 125-135.
[54] Bailey BA, Bae H, Strem MD, et al. Developmental expression of stress response genes in Theobroma cacao leaves and their response to Nep1 treatment and a compatible infection by Phytophthora megakarya[J]. Plant Physiol Biochem, 2005, 43(6): 611-622.
[55] Ashihara H, Monteiro AM, Moritz T, et al. Catabolism of caffeine and related purine alkaloids in leaves of Coffea arabica L.[J]. Planta, 1996, 198(3): 334-339.
[56] Mazzafera P.Catabolism of caffeine in plants and microorganisms[J]. Front Biosci, 2004, 9: 1348-1359.
[57] 余有本. 茶树中咖啡碱合成酶基因的抑制及在其它生物体中的表达[D]. 安徽: 安徽农业大学, 2004: 79.
[58] 张广辉, 梁月荣, 陆建良, 等. 茶树咖啡因合成酶基因RNA干涉表达载体构建[J]. 茶叶科学, 2006, 26(4): 243-248.
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