采用邻苯二甲醛作为柱前衍生化试剂,结合反相高效液相色谱,优化了色谱条件,实现对茶汤中17种一级氨基酸的精确定性和定量分析。17种氨基酸的保留时间和峰面积的RSD分别在0.02%~0.70%与0.11%~1.15%之间,经方法学考察,该方法具有良好的稳定性和重现性。
In this paper, a reversed-phase high performance liquid chromatography combined with precolumn derivatization with O-phthalaldehyde and ?uorescence detection was set up for the qualitatively and quantitatively analysis of seventeen kinds of free amino acids in tea. The RSD of the retention time and peak area of the 17 kinds of free amino acids were 0.02%~0.70% and 0.11%~1.15%, respectively. With validation, this method was proved to be accurate, stable and reproducible for the analysis of free amino acids in tea.
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