为了研究茶多酚对苯并[a]芘致日本青鳉鱼后代畸形的拮抗及其可能机理,本研究采用正在产卵的青鳉鱼为模式生物,采用腹腔注射法研究了茶多酚对苯并[a]芘致其后代发育畸形的影响,并用逆转录-实时荧光定量PCR法检测肝脏组织内的CYP酶（CYP1A1、CYP1B、CYP2A、CYP2C、CYP2D、CYP3A）和GST基因（mGST、GST-a）的mRNA表达情况。结果表明,单独注射苯并[a]芘组日本青鳉鱼后代畸形率达到15.13%,显著高于对照;茶多酚拮抗组后代畸形率有所下降,除茶多酚最低剂量组（20μg/g BaP+2μg/g TP）外,其他组与BaP组间均存在显著差异。实时荧光定量PCR结果表明,苯并芘试验组对CYP1A1、CYP1B、CYP2A、CYP2C、CYP2D有轻微诱导,并显著抑制雌鱼mGST和GST-a的表达,同时茶多酚可明显抑制CYP1A1基因和谷胱甘肽-S转移酶基因mGST和GST-a的表达。表明茶多酚的抗畸变机理可能与抑制CYP1A1基因表达以及加速苯并芘代谢有关。
The effects of green tea polyphenols on the malformations of the fertilized eggs of Japanese medaka (Oryzias latipes) induced by benz[a]pyrene was investigated by using the biological model of spawning Japanese medaka (Oryzias latipes) with the method of intraperitoneal injection, and the real-time PCR was used to detection the mRNA expression of CYP enzymes (CYP1A1, CYP1B, CYP2A, CYP2C, CYP2D, CYP3A) and the GST gene (mGST, GST-a) in the liver medaka. The results showed that the malformation rate of the fertilized eggs of Japanese medaka in the treatment of injection of benzo[a]pyrene which was significantly higher than the DMSO control. The malformation rate in the treatments of tea polyphenols was decreased, the treatments except the lowest dose of tea polyphenols (20μg/g BaP +2μg/g TP) were significantly different compared to the BaP treatment. Real-time quantitative PCR results showed that the benzo[a]pyrene model group slightly induces the CYP1A1, CYP1BCYP2A, CYP2C, CYP2D mRNA expression and significantly inhibits the mGST and GST-a mRNA expression of the female medaka, while tea polyphenols can significantly inhibit the expression CYP1A1 mRNA gene and glutathione-S transferase (GST) gene mGST and GST-a. The antagonistic mechanism of green tea polyphenols on the malformation of the fertilized eggs of Japanese medaka induced by benzo[a]pyrene might be correlated with the inhibition of CYP1A1 mRNA gene expression and the acceleration of the excretion of metabolic product of benzopyrene.
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