通过盆栽试验研究氟了对茶树新梢生长和品质成分含量的影响。适量增加土壤氟水平能提高新梢产量,但在高氟水平下新梢产量明显下降。茶多酚、咖啡碱及儿茶素(EGCG等)的含量随土壤氟水平的增加而逐渐降低,高氟水平下新梢的游离氨基酸含量也显著下降,苯丙氨酸解氨酶(PAL)、谷氨酸合成酶(GOGAT)的活性也随土壤和茶树氟浓度的增加而下降,说明氟可能抑制茶树儿茶素的合成代谢和氮素代谢。上述结果表明,氟对茶树生长和品质成分的影响与氟水平有关,虽然低浓度氟对茶树生长有一定的促进作用,但高浓度氟抑制茶树生长和茶多酚、氨基酸等品质成分的代谢。
Soil experiment was conducted to investigate the impact of fluoride on yield of young shoots and chemical ingredients related with tea quality. Production of young shoots was improved by modest increase in the soil fluoride levels while significantly decreased by higher F levels. The concentrations of total polyphenols, caffeine and catechins (EGCG, etc) were reduced by fluoride application. The concentrations of total free amino acid and some major components (theanine, glutamate and glutamine) in the young shoots were also decreased significantly by the highest F application. Activity of phenylalanine ammonia-lyase (PAL) and glutamate synthase (GOGAT) decreased with increasing fluoride levels. The results indicated that, though tea plant growth was improved by low level of fluoride, high levels of this element were detrimental to both growth and metabolism of chemical components such as catechins, caffeine and free amino acids.
[1] Rao GS.Dietary intake and bioavailability of fluoride[J]. Annual Review of Nutrition, 1984, 4: 115~136.
[2] Fung KF, Zhang ZQ, Wong JWC, et at. Fluoride contents in tea and soil from tea plantations and the release of fluoride into tea liquor during infusion[J]. Environmental Pollution 1999, 104: 197~205.
[3] 白学信, 方进良, 吴金生, 等. 饮茶型氟中毒的氟骨症调查[J]. 地方病通报, 1994(9): 65~66.
[4] 曹进, 赵燕, 列箭卫, 等. 西藏高海拔地区儿童的砖茶型氟中毒[J]. 中国公共卫生, 2000, 16(2): 174~175.
[5] Miller GW.The effect of fluoride on higher plants: with special emphasis on early physiological and biochemical disorders[J]. Fluoride, 1993, 26: 3~22.
[6] Davison AW, Takmaz-Nisanciolu S, Bailey IF.The dynamics of fluoride accumulation by vegetation. In: Susheela AK, ed. Fluoride toxicity. New Dehli: International Society for Fluoride Research, 1985. 0~46.
[7] Ruan J, Wong MH.Accumulation of fluoride and aluminium related to different varieties of tea plant[J]. Environmental Geochemistry Health, 2001, 23: 53~63.
[8] 王小平, 刘鹏, 罗虹, 等. 氟铝交互处理对茶叶品质的影响[J]. 茶叶科学, 2009, 29(1): 9~14.
[9] 王新超, 杨亚军, 陈亮. 茶树谷氨酸合酶的提取与活性测定[J]. 中国茶叶, 2004, 5: 10~11.
[10] Edwards R, Kessmann H.Isoflavonoid phytoalexins and their biosynthetic enzymes. In: Gurr S J, ed. Molecular Plant Pathology. Volume II A Practical Approach. McPherson M J and Bowles D J. Oxford University Press, Oxford. 1992: 45~62.
[11] Ruan JY, Gerendás J, Härdter R, et al. Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis) plants[J]. Annals of Botany, 2007, 99(2): 301~310.
[12] 钟萝. 茶叶品质理化分析[M]. 上海科学技术出版社, 1989: 258, 333.
[13] Waters公司. AccQ–Tag氨基酸分析方法[M]. Milford, MA, USA.
[14] 于海宁, 沈生荣, 臧荣春, 等. 多酚中儿茶素类的HPLC分析方法学考察[J]. 茶叶科学, 2001, 21(1): 61~64.
[15] 石元值, 杨亦扬, 阮建云, 等. 用离子色谱法同时测定茶叶中的F-、Cl-、SO42-、NO3-[J]. 食品科学, 2008, 29(3): 383~385.
[16] 宛晓春. 茶叶生物化学(第三版)[M]. 北京: 中国农业出版社, 2003.