为在分子水平上鉴定“玉绿”和“玉笋”的父本,本研究采用EST-SSR标记对其母本和4个可能父本进行了分析。结果表明,筛选的16对核心引物共检测到34个等位位点,每个引物1~3个,平均2.12个。玉绿与薮北实生单株及4个可能父本的相同位点数分别占玉绿扩增总位点数的72.22%、44.44%、55.56%、72.22%、61.11%,最大为薮北实生单株和湘波绿,最小为福鼎大白茶;玉笋分别为73.91%、43.48%、52.17%、60.87%和47.83%,最大为薮北实生单株,最小也为福鼎大白茶。8份供试材料间相似系数变幅为0.19~0.85,平均0.52。根据相似系数UPGMA聚类,在相似系数0.61处将参试8份材料分为4类：第I类为薮北、薮北实生单株、玉笋;第II类为龙井43;第III类包括湘波绿、玉绿、槠叶齐;第IV类为福鼎大白茶。参试材料的相似系数和亲缘关系树状图在分子水平上显示了玉绿和玉笋杂交亲本间的遗传差异较大,推测湘波绿可能是玉绿的父本,而玉笋的父本还有待进一步验证。
EST-SSR primers were used to identify the male parents for Yulv and Yusun among Yabukita seeding and other 4 possible male parents. Totally 34 alleles were amplified using 16 core EST-SSR primers, the number of alleles per primer ranged from 1 to 3, on average of 2.12. The percentage of common alleles with Yulv is 72.22%, 44.44%, 55.56%, 72.22%, 61.11% each other, the maximum is Yabukita seeding and Xiangbolv, the minimum is Fuding Dabaicha, The percentage of Yusun is 73.91%, 43.48%, 52.17%, 60.87% and 47.83%, respectively, the maximum is Yabukita seeding, the minimum is Fuding Dabaicha.The similarity coefficient among different cultivars varied from 0.19 to 0.85, on average of 0.52. The 8 accessions were classified into 4 groups based on the UPGMA method with the similarity coefficient at 0.61: The first group is Yabukita, Yabukita seeding and Yusun. The second is Longjing 43. The third is Xiangbolv, Yulv and Zhuyeqi. The last is Fuding Dabaicha. The results of the similarity coefficient and dendrogram showed that the accessiongs have more genetic diversity and heterosis, Xiangbolv is the possible male parent of Yulv and which of Yusun should be identified furtherly.
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