以森林和蔬菜土壤作对照,采用实验室培养研究了高产、中产和低产等三种茶园土壤N2O的排放水平,试验设不加氮（对照）与加氮[200 mg/kg,(NH4)2SO4]二处理,在25℃恒温培养0、1、3、7和14 d时分别取样检测N2O释放量。另外,选择两种茶园土壤研究了土壤含水量与加氮对N2O排放影响的交互作用。结果表明,对于不加氮的对照土壤组,高产茶园具有较高的N2O排放量,14 d内平均日排放量高达11.26 mg/(kg·d)（以纯氮计）,显著高于其它四种土壤;但对于加氮处理组,以菜园土壤的N2O排放水平最高,极显著高于茶园和林地土壤;所有五种土壤加氮后,N2O排放量均有明显提高。茶园土壤N2O排放水平随着土壤含水量的提高而增加,并与施氮存在显著的正交互作用,当土壤含水量较高时施氮具有刺激N2O排放的作用。文章根据土壤培养期间NH4+和NO3-含量的变化就N2O形成机理进行了讨论。
Using forest and vegetable soils as controls, three types of tea garden soils, namely high, middle and low productive tea garden soils, were collected to study N2O emission under laboratorial incubation condition. There were two treatments: (I) control soils without addition of any chemicals. (II) N treatment, adding 200 mg N in form of (NH4)2SO4 per 1 kg soil. The soils were continuously incubated at 25℃ for 14 days. After 0, 1, 3, 7 and 14 days incubation, the air in head space of the incubated bottles were collected, respectively, for measuring N2O concentrations. The interaction of soil moisture and N addition on the N2O emission was also studied. The results showed that in a group with natural soils, the soil of high production tea garden had the highest mean N2O emission rate during 14 days incubation period. It reached to 11.26 mg/(kg·d), significantly higher than other four soils. However, in the other group with N addition, vegetable soil showed the highest N2O emission rate, very significantly higher than those in tea and forest soils. The N2O emission rate in N added soils was higher than that in natural soils. The N2O emission rate was increased with the increase of soil moisture content. There was a significant interaction between soil moisture content and N added. N addition to high moisture content soils could stimulate the N2O emission. The paper also discussed the possible N2O production mechanism based on the change of NH4+ and NO3- concentration during the incubation period.
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