将DL-茶氨酸乙酰化为N-乙酰-DL-茶氨酸,利用本实验室筛选的有较高氨基酰化酶活性的真菌刺孢小克银汉霉9980（Cunnighamella echinulata 9980）对DL-茶氨酸进行拆分并对拆分条件进行摸索。结果表明：反应最适温度50℃,最适pH7.0,底物浓度0.5mol/L,湿菌体量4g/100βml,拆分时间30h,拆分率可达92％。产物经JK008阳离子交换树脂氨性柱分离,L-茶氨酸收率84.3％,[α] =8.1(c=2,H2O), 符合JP2000药典。
An enzymatic method for the optical resolution of DL-theanine by aminoacylases from Cunnighamella echinulata 9980 was developed. The optimum temperature, pH and substrate concentration were: 50℃, pH7.0, and 0.5mol/L N-Ac-DL-theanine. The conversion rate against N-Ac-L-theanine was 92%. Productions were purified on JK008 ion exchange resin. The yield of L-theanine was 84.3%, [α] =8.1(c=2,H2O), complied with JP2000。
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