利用高通量筛选法寻找普洱茶降血糖血脂作用的活性成分的研究。实验以SMMC-7721细胞为模型,确定普洱茶各样品的合适筛选浓度为50βμg/ml,并以此浓度对激活PPARα、PPARδ、PPARγ受体的活性进行筛选。结果发现,普洱茶醇提物对PPARδ受体有激活作用,有潜在的降血糖和降血脂作用的可能。
Discovering the active substances of Pu’er Tea on blood glucose depressing and blood lipid-depressing function was studied by high throughput screening method. In this experiment, SMMC-7721 cells were used as the model, the screening concentration of samples was established at to 50βμg/ml, and the activity of Pu’er Tea on activing PPARα、PPARδ、PPARγ was screening. The result showed that Pu’er Tea ethanol extract can active PPARδand may be have the function of lowering blood glucose and blood lipid depressing function.
[1] 邵丙扬. 警惕世界面临糖尿病灾难[J]. 内科急危重症杂志, 2004, 10(1): 1~2.
[2] 何宇纳,林海.中老年人的肥胖及其影响因素[J]. 中国食物与营养, 2000, (3) :8~9
[3] Vaccitolo J A, Connolly H M, Rubenson Ds, et al. Operation for anorexigen-associated Vavular heart disease[J]. Thorac Cardiovasc Surg, 2001, 122(4): 656.
[4] 倪德江, 谢笔均, 宋春和. 不同茶类多糖对实验型糖尿病小鼠治疗作用的比较研究[J]. 茶叶科学, 2002, 22(2): 160~163.
[5] 王蓓,邓红,苏宁,等.茶多酚对糖尿病大鼠活性氧表达的影响[J].东南大学学报(医学版),2002,21(1):40~43.
[6] 张玉叶, 刘彦平, 谢六生, 等. 茶色素对Ⅱ型糖尿病患者血糖、血脂、血液流变学的影响[J]. 中草药, 2001, 32(7): 632~633.
[7] 周红杰, 秘鸣, 韩俊, 等. 普洱茶的功效及品质形成机理研究进展[J]. 茶叶, 2003, 29(2): 75-77.
[8] Keith J W, Richard A M, Michael W.Recent advances in discovery technologies[J]. Neurotransmissions, 1997, 53(3): 1~5.
[9] Braissant O, Foufelle f, Scotto C, el al. Differential expression of peroxisome proliferators-activated receptors(PPARs):tisue distribution of PPARa, δand γ in the adult rat[J].Endocrinology, 1996, 137(1): 354~366.
[10] Okuno A, Tamemoto H, Tobe K, et a1.Troglitazone increases the number of small adipocytes without the change of white adipose tissue mass in obese Zucker rats[J]. Clin Invest, 1998, 101(6): 1354~1361.
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