用易感茶芽枯病的6个品种和3个抗病品种进行间栽和净栽试验,并对10个品种种质的遗传多态性、亲缘关系进行RAPD分析。结果表明:10个茶树品种聚类结果分为2个复合组和3个独立组,多态性谱带为110条(占89.43%),间栽比净栽茶芽枯病叶片受害面积,受害面积百分率,发病率及病情指数间栽后均有显著下降。易感品种下降幅度更大,防治效果达28.5%~73.77%,利用品种的多样性混合间栽是控制茶芽枯病的有效途径。
Field experiments of mixed and single-cultivar-planting of 6 susceptible cultivars and 3 tea bud blight-resistant cultivars, and the genetic diversity, and the relationship of 10 tea cultivars were investigated by RAPD. The results demonstrated that 10 tested tea cultivars can be classified into 2 complex groups and 3 simple groups, a total of 110 polymorphic DNA bands were amplified (89.43%). The disease incidence infected leaf-area percentage disease index and infected leaf area in the mixed planting plots were significantly lower than those in the single-cultivar-planting plots. The susceptible cultivars showed significant greater decreasing range the control efficiency gained 28.54%~73.77%. It suggests that inter-planting of tea cultivars with different genetic background and morphological characteristic is an effective approach for tea bud blight control.
[1] 谭济才. 茶树病虫防治学[M]. 北京: 中国农业出版社, 2002: 217~221.
[2] 王晓鸣. 作物遗传资源的抗病虫多样性与农业可持续发展[J]. 中国农业科学导报, 2002, 2(5): 67~70.
[3] Clark M.S(顾红雅译) 植物分子生物学实验手册[M].北京高等教育出版社..1999.6-7
[4] Nei M and Li W H. Mathematical model for studying genetic variation in terms of restriction endonuc leases[J]. Proc Natl Acad Sci USA, 1997, 76(10): 5269~5273.
[5] 中国茶树品种志编委会. 中国茶树品种志[M]. 上海科学技术出版社, 2001: 15~112
[6] 胡淑霞. 皖南茶树病害的发生及防治[J]. 茶业通报, 1999, 16(1): 20~21.
[7] 赵和涛. 茶树茶枯病的症状及防治措施[J]. 热带作物科技, 1991(3): 81~82.
[8] 高旭辉. 茶芽枯病的综合防治[J]. 蚕桑茶叶通讯, 1989.
[9] 卢良恕. 21世纪的农业和农业科学技术[J]. 科学导报, 1996(12): 1~8.
[10] 戴小枫, 叶志华. 浅析我国农作物病虫草鼠害成灾特点与减灾对策[J]. 应用生态学报, 1999(10): 119~122.
[11] Staskwicz B J,Ausube(FM, Baker), Ellis JG. et al. MOlecular geneties of plant disease resistance[J]. science1995(268): 661~667.
[12] Wolfe M S.The current status and prospects of multilane cultivara and variety mixture of disease resistance[J]. Annual Review of phytopathology, 1985(23): 251~273.
[13] Garrett R A, Nunde C C.Epidemiology in mixed host populations[J]. phytothology, 1999(89): 984~990.
[上接第252页]
[4] Holger Schmidt, Ludwig Niessen, Rudi F.Vogel.AFLP analysis of Fusarium species in the section Sporotrichiella-evidence for Fusarium langsethiae as a new species[J]. International Journal of Food Microbiology, 2004(95): 297~304.
[5] Carlos Polanco, Marı´a Luisa Ruiz, AFLP analysis of somaclonal variation in Arabidopsis thaliana regenerated plants[J]. Plant Science, 2002(162): 817~824.
[6] Mireille Fargette, Virginie Lollier, Mark Phillips.AFLP analysis of the genetic diversity of Meloidogyne chitwoodi and M. fallax, major agricultural pests[J]. C R Biologies, 2005(328): 455~462.
[7] 黄建安. 茶树分子遗传图谱构建及多酚氧化酶基因的SNP研究[A]. 湖南农业大学博士论文, 2004.
[8] 黄建安, 黄意欢, 罗军武, 等. 茶树基因组DNA的高效提取方法[J]. 湖南农业大学学报, 2003, 29(5): 402~407.
[9] Massimo L, Fabrizio G, Serena I, et al. AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plant[J]. Chemosphere, 2003, 52(7): 1183~1188.